FastQCFastQC Report
Wed 25 May 2016
SRR1781038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257335
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT7442028.91950181669808No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG94033.6539918782909435No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC51892.016437717372297No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC20240.7865234033458333No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT19620.7624302951405755Illumina PCR Primer Index 3 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC11560.4492198884722249No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT8140.3163191948238677No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT7740.3007752540462821No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG6230.2420968776108963No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC5950.23121611906658635No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC4830.18769308488934658No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT4800.18652728933102766No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTAATGCATCTCGTATGCCGT4420.1717605455923213No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT3860.1499990285037014No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT3790.1472788388676239No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCAGT3780.14689024034818426No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGAATCTCGTATGCCGT3630.14106126255658966No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC3630.14106126255658966No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG3350.1301805040122797No Hit
CTTATACACATCTCCGAGACCACGAGACTTTAATGCATCTCGTATGCCGT3180.1235743291818058No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGACGT3090.12007694250684905No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG2670.10375580469038413No Hit
CTTATACACATCTCCGAGCCCACGAGACATTAATGCATCTCGTATGCCGT2620.10181281209318592No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG150.00221980570.024886
TAGGGCG150.00221980570.024884
GTCTTAA207.918251E-569.9976749
CGTCAAC207.918251E-569.9976748
CCTTGAA207.918251E-569.9976756
GCCCGAA207.918251E-569.9976744
AGCCCGA150.002223230769.99766543
GCAACAC150.002223230769.99766535
GACGTAT150.002223230769.99766546
CACGAAT150.002223230769.99766539
CAACACG150.002223230769.99766536
ATCCGAT150.002223230769.99766512
AAGCCCG150.002223230769.99766542
CTGCCGT150.002223230769.99766545
AGTCTAC150.002223230769.99766548
CGAGCAT150.002223230769.99766518
ACGTAGA150.002223230769.9976657
GCCGTAA252.835288E-669.99766546
CAACTCG1450.065.1702436
AGGGGGG81700.065.1141170
Produced by FastQC (version 0.11.5)