FastQCFastQC Report
Wed 25 May 2016
SRR1781037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241440
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7511831.112491716368456No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGT150.00222316169.99689548
TGCGGGT252.3877657E-455.9975175
GCTGTAC200.006940949752.50854565
GATACAT200.006946639652.4976738
GAATGGT200.006946639652.4976721
TGTGCGC200.006946639652.497673
ATCGTAA200.006946639652.4976750
TGCGCGT200.006946639652.497675
TGATTGC200.006946639652.4976760
CTTGACG200.006946639652.4976727
GTCCGCC200.006946639652.4976723
CGGCTGC200.006946639652.4976736
GTGCGCG200.006946639652.497674
TGCACTA305.865417E-446.67426312
GCACTAC305.865417E-446.67426313
GCCATGC404.585176E-543.748068
GCGCTGC350.001252795740.0065115
AAGCGTT350.001254072939.99822636
CCCATAG350.001254072939.9982261
CGCTCAT501.7048583E-435.005770
Produced by FastQC (version 0.11.5)