FastQCFastQC Report
Wed 25 May 2016
SRR1781037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241440
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4898020.286613651424783No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG64982.691351888667992No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC27231.1278164347249835No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC13280.5500331345261763No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT12400.513585155732273RNA PCR Primer, Index 34 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC8620.3570245195493704No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4810.19922133863485753No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4650.1925944333996024No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG4060.16815772034459908No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3850.1594599072233267No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT3710.15366136514247847No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC3680.15241882041086813RNA PCR Primer, Index 34 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTAGTATGCCGT3650.1511762756792578No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGACGT3620.14993373094764745No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCAAGGGATCTCGTATGCCGT2960.12259774685222001No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC2800.11597084161696489No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT2580.10685884691848907No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG2580.10685884691848907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCCC150.002223683369.9927526
GCAAACG150.002223683369.9927518
AAGCGTC150.002223683369.9927519
ACGATTG150.002223683369.9927514
CCGAATT150.002223683369.9927547
AGGGGGG52250.064.165670
AGCCGTC550.063.62977245
TCTACGA501.4551915E-1162.99347311
TGCCGAC850.061.7583145
AAGCCGT700.059.99378644
GCCGACT700.059.99378646
ACTCCGA352.9681905E-759.99378611
CGAATGC650.059.22463241
ACGTATG650.059.22463240
TCTGCAA308.365272E-658.32729353
CTGCAAG308.365272E-658.32729354
TCCCACG308.365272E-658.32729318
CACCGAG850.057.6410912
ATCGTAT1550.056.4457739
CGTCTTA2050.056.3356348
Produced by FastQC (version 0.11.5)