FastQCFastQC Report
Wed 25 May 2016
SRR1781036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379996
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6924918.22361288013558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC308.369716E-658.33289725
TAGACCG200.00694868152.4996035
ATGCGAA200.00694868152.49960323
GTCCAAC404.57849E-543.766951
CATCGTA404.5891764E-543.7496724
TCACTAG350.001254754639.999716
TCGTACT350.001254754639.999726
CTTCAGA552.9829246E-431.8305071
TAACCGG450.004305025631.11087822
GAGCATG450.004305025631.11087848
ATGCAGT450.004305025631.1108784
CACGTCT5150.028.54347270
CTACGGG500.00720465727.99978838
ACATGTT657.94983E-426.92287329
ACCCGAT657.94983E-426.92287327
GCATGTA808.6483866E-526.2532582
TCTCGGT1207.383096E-826.24980443
ATTAGAG808.656133E-526.2498023
TATTAGC700.001224157525.0031012
GTCTCGG700.001225097324.99981142
Produced by FastQC (version 0.11.5)