FastQCFastQC Report
Wed 25 May 2016
SRR1781036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379996
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4336211.411172749186834No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG63481.67054390046211No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC28500.7500078948199455No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC14470.38079348203665303No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT12160.32000336845651006TruSeq Adapter, Index 10 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC7410.19500205265318582No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT5480.1442120443373088No Hit
CATATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT4700.12368551247907873No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG4190.11026431857177443No Hit
CTTTACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTC3840.10105369530205581No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCCC150.002220937370.022387
AGGGGGG50100.063.21879670
AAACTCG1100.060.45000536
AACTCGT850.057.64272337
GGCTAAA1700.055.58405332
TCTTTAT2850.054.0523641
CGTCTTA407.474846E-752.4960548
CTCGAAA200.006950540552.4960539
TATGCCG70200.050.6016143
AATCTCG71000.050.5736636
GCTAATC71250.050.54356833
CGTATGC71250.050.4453341
GTATGCC70500.050.43592542
TCGTATG71850.050.41374640
GCCGTCT69600.050.33385546
CGGCTAA73400.050.25507731
GGCTAAT73000.050.24279832
TCTCGTA70500.050.2373638
CTCGTAT70650.050.22976739
ATGCCGT70950.050.1653644