FastQCFastQC Report
Wed 25 May 2016
SRR1781035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128164
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7830761.099060578633626No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTT150.002220433269.9996126
CGGCGAG207.9020276E-569.9996122
GCGCGTC301.0170697E-769.999616
TCACACC150.002220433269.999613
CCGGCGA207.9020276E-569.9996121
GTCTCGG150.002220433269.9996142
GGTCCAA150.002220433269.9996121
ATAGATT150.002220433269.9996114
GTTAGCT150.002220433269.9996121
CTAACTT150.002220433269.9996122
GGGACGA150.002220433269.9996126
TTGGTCC150.002220433269.9996119
GGAGTTC150.002220433269.9996138
AGATTGG150.002220433269.9996116
TAGATTG150.002220433269.9996115
TTAGTCA150.002220433269.9996113
GGCGCGT207.9020276E-569.999615
GGTCTCG150.002220433269.9996141
CGCGTCT207.9020276E-569.999617
GCGCTGC150.002220433269.9996115
Produced by FastQC (version 0.11.5)