FastQCFastQC Report
Wed 25 May 2016
SRR1781035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128164
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT5059439.47598389563372No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG70805.52417215442714No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC27722.162853843512999No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC13981.0907899254080708No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT10310.8044380637308449RNA PCR Primer, Index 14 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC7670.5984519833962736No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4940.38544365032302363No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT4130.3222433756749165No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG4080.31834212415342844No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT4050.31600137324053557No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCACATCTCGTATGCCGT3890.3035173683717736No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3850.30039636715458323No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTCCCATCTCGTATGCCGT3630.2832308604600356No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC3480.27152710589557133No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT3270.2551418495053213No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3020.23563559189788086No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGATCCCATCTCGTATGCCGT3010.23485534159358323No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCAGT2680.20910708155176178No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCAATCTCGTATGCCGT2520.19662307668299991No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG2240.17477606816266658No Hit
CTTATACACATCTCCGAGCCAACGAGACCGGTTCCCATCTCGTATGCCGT2190.17087481664117848No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG2120.16541306451109516No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT2120.16541306451109516No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1990.1552698105552261No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1980.1544895602509285No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCATCTCGTATGCCGTC1780.1388845541649761No Hit
CTTATACACATCTCCGAGACCACGAGACCGGTTCCCATCTCGTATGCCGT1730.134983302643488No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGA1350.10533379108017853No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTAAGCCGT1310.10221278986298804No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC207.874121E-570.049594
CCTTATA301.014796E-770.022251
CCAAGGT150.002217585770.022258
AACGTAT150.002221022369.9949339
AACCGAG207.9046455E-569.9949312
ACCGGTA500.069.9949327
AATTTGC150.002221022369.9949336
TCACCGA455.456968E-1269.9949311
ATCTAAG207.9046455E-569.9949310
GACCGCT150.002221022369.9949326
ACCGAGA150.002221022369.9949313
CAACACG252.8278228E-669.9949336
AACGGTA150.002221022369.9949327
AACGGAT252.8278228E-669.9949327
AAACGAG207.9046455E-569.9949320
GCCGACA252.8278228E-669.9949346
CACTACG150.002221022369.9949352
GCTTAAA150.002221022369.9949366
CAAATCG301.0175427E-769.9949336
GAACGGA252.8278228E-669.9949326
Produced by FastQC (version 0.11.5)