FastQCFastQC Report
Wed 25 May 2016
SRR1781034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323807
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6396119.752815720475468No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGA200.00694827752.49861570
TGACGAC200.00694827752.49861569
ACTACGT200.00694827752.4986153
ATCGAGC200.00694827752.49861531
TGCGCGT352.0844505E-549.9986765
GACGATA305.8738806E-446.66543241
CGATAAC305.8738806E-446.66543243
CCGACGC602.592642E-740.83225370
GATAACG350.001254596939.99894344
GCGCGTC459.188697E-538.887866
CGCGTCT459.188697E-538.887867
GTGCGCG501.7080421E-434.9990734
TGTGCGC450.004304494731.1102873
ATAACGT450.004304494731.11028745
GTGACGA450.004304494731.11028739
GTATTAC703.4472432E-530.04561
GCATGGG703.4838147E-529.99920730
ACGATAA500.007203772727.9992642
TCTGGCG755.5768665E-527.9992611
TGACGAT657.948155E-426.92236540
Produced by FastQC (version 0.11.5)