FastQCFastQC Report
Wed 25 May 2016
SRR1781034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323807
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT141454.368342870907671No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT49001.513247088543484No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG13390.4135179288897399No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT12260.3786205980723085No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT10780.33291435947956655No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT7720.2384136229297081No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT7410.22884001890014732No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG6950.21463402582402483No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC5950.18375143218028023No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGAGTATGCCGT5930.18313378030740535No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT5210.16089831288390924No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT4680.14453053825272463No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCCT4130.1275451117486651No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT4020.12414802644785321No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG3810.11766268178266684No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG150.002219743670.029515
AAATCCG252.8370905E-669.9970743
ACGATAC207.9216756E-569.9970622
AATTCGC150.002223827269.9970627
ATGACCG150.002223827269.9970644
CGACTTA600.069.9970648
ATACGAC150.002223827269.9970645
TCGACTA150.002223827269.9970638
TCGCGAC150.002223827269.9970630
AGGGGGG27450.065.407170
CCGACTT501.4551915E-1162.99736447
TGCCGAC850.061.7621245
CGACATA700.059.99748648
CGTATGC24600.057.61953741
GCCGTCA5850.057.4334946
CTTATGC8200.056.76591551
TATGCCG32400.056.7105943
GCCGTCT14950.056.6531446
TGCCGTC20300.056.3769545
ATGCCGT30950.056.3142844
Produced by FastQC (version 0.11.5)