FastQCFastQC Report
Wed 25 May 2016
SRR1781033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152035
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5052333.231163876738904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTT150.002221264969.9993447
TCCGTTC150.002221264969.9993448
TTCGCTA150.002221264969.99934440
ACGTTAG200.006940752752.49950869
CGTTCTT200.006940752752.49950810
AATTGCT200.006940752752.49950836
CGCTACT200.006940752752.49950842
GAGTTAG200.006940752752.49950830
TGGTGCG200.006940752752.4995082
CCGTTCT200.006940752752.4995089
TATCCCA200.006940752752.49950818
AGTAATC404.5761753E-543.74958859
CCTCGCC350.001252392239.99962632
GTGCTGA350.001252392239.9996266
CTCGCCC350.001252392239.99962633
CTTCGCT400.00241296834.99967239
GTAATCA400.00241296834.99967260
CCTTCGC652.0922407E-532.3073938
GGCGCTG552.9815003E-431.81788314
GCACCTG450.004297020431.11081767
Produced by FastQC (version 0.11.5)