FastQCFastQC Report
Wed 25 May 2016
SRR1781033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152035
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3138520.643272930575197No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG49453.252540533429802No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC19131.258262899990134No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT10040.6603742559279113No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC9070.5965731574966291No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT8560.5630282500739962Illumina PCR Primer Index 11 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC4690.30848159963166377No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3930.25849311013911275No Hit
CATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3170.20850462064656164No Hit
CTTTACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2610.17167099680994508No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG2510.16509356398197783No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT2160.14207254908409248No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC2140.14075706251849904No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2090.13746834610451542No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT2030.13352188640773507Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTCAATCTCGTATGCCGT1910.12562896701417436No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCAGT1880.1236557371657842No Hit
CTTATACACATCTCCGAGCCAACGAGACGACGCTCCATCTCGTATGCCGT1840.12102476403459729No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT1670.10984312822705299No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCG1550.10195020883349229No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCG150.002218532470.021065
GACGTAT150.002221430269.99802446
TAGCGAA150.002221430269.9980247
AACGGAT150.002221430269.99802440
GAACGGA150.002221430269.99802439
GCAATTA150.002221430269.99802436
GACGATA150.002221430269.99802429
AACACCG252.8296163E-669.99802410
AGGGGGG35700.065.1942470
CGTCTAC501.4551915E-1162.99822248
GCCGTAT352.9592593E-759.9983146
GAAGACG308.346698E-658.33168826
GCCGCAC308.346698E-658.33168829
CGCCGCA308.346698E-658.33168828
TGACGTC1200.055.41510445
ATCACCG950.055.261610
TATGACG1600.054.6859643
ATATCCG452.6833732E-854.4429110
TGCCGTA452.6833732E-854.4429145
TATCCGA452.6833732E-854.4429111
Produced by FastQC (version 0.11.5)