FastQCFastQC Report
Wed 25 May 2016
SRR1781032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119151
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6511054.644946328608235No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGC150.002220052869.9995815
CGGTGCC252.3823898E-455.99966416
CTACACC252.3823898E-455.9996644
TCTTGAA200.00693699452.4996872
CGGCCGG200.00693699452.49968718
CTAAGTT200.00693699452.4996874
GGCCCCT200.00693699452.4996876
GCTCTTA200.00693699452.49968752
GGTGCGC200.00693699452.4996873
GCGGCCG200.00693699452.49968717
TTCGGGG200.00693699452.49968723
CCAACCT200.00693699452.49968758
AAGATAA200.00693699452.49968715
TGAGCTG200.00693699452.49968731
TGAGCCT200.00693699452.49968717
GTGCGCG200.00693699452.4996874
TGCCGGC200.00693699452.49968719
GGGGTTA305.8582675E-446.6663866
GGTGCCG305.8582675E-446.66638617
GCCGGCG404.5700945E-543.7497420
Produced by FastQC (version 0.11.5)