FastQCFastQC Report
Wed 25 May 2016
SRR1781032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119151
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT4337036.399190942585456No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG61855.190892229188173No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC25322.1250346199360477No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC11991.0062861411150557No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT10710.8988594304705793TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC7150.6000788914906295No Hit
CATATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT5580.468313316715764No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT5250.440617367877735No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG3510.29458418309539997No Hit
CTTTACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC3250.2727631324957407No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGAAAGATCTCGTATGCCGT3160.265209691903551No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTC2970.24926353954226152No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC2960.2484242683653515No Hit
CTTATACACATCTCCGAGCCAACGAGACGGAGTAAGATCTCGTATGCCGT2930.2459064548346216No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT2850.23919228541934184No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGCTCTCGTATGCCGT2810.23583520071170194No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCAGT2530.21233560775822274No Hit
TATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCT2530.21233560775822274No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG2250.18883601480474355No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGACGT2180.18296111656637376No Hit
CTTATACACATCTCCGAGACCACGAGACGGAGTAAGATCTCGTATGCCGT2170.1821218453894638No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAATCTCGTATGCCGTC1690.14183682889778515No Hit
CTTATACACATCTCAGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1560.13092630359795554No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG1560.13092630359795554No Hit
CTTATACACATCTCCGAGCACACGAGACGGAGTAAGATCTCGTATGCCGT1500.12589067653649572No Hit
TCTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT1400.11749796476739599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC455.456968E-1269.9924548
CGAGACT207.903755E-569.9924523
GCCGTAT207.903755E-569.9924546
CTCACGT150.002220950369.9924437
ACTGCAT150.002220950369.9924453
CGTATCA150.002220950369.9924441
GCGTTAA150.002220950369.9924429
CCGTCAA150.002220950369.9924447
AGACGCG150.002220950369.9924425
AATGACG150.002220950369.9924443
TACGAGA150.002220950369.9924413
GACGCGT150.002220950369.9924426
AACTCCC150.002220950369.9924410
CGCGTTA150.002220950369.9924428
GTAAGAC150.002220950369.9924442
GACGACA150.002220950369.9924446
CGTCAAC150.002220950369.9924448
TGCCGTA150.002220950369.9924445
ACGCGTT150.002220950369.9924427
AACTCGT750.065.3262837
Produced by FastQC (version 0.11.5)