FastQCFastQC Report
Wed 25 May 2016
SRR1781031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263115
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8418631.995895330938946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA150.002223064469.99943533
TAGCGTA150.002223064469.99943532
GTTCTCG200.00694633452.49957361
GCGCGTT200.00694633452.49957357
ATTCGCA200.00694633452.49957334
GCTAGAT200.00694633452.49957370
CGCGTTC200.00694633452.49957358
CGCAACT200.00694633452.49957340
ATTTGCG200.00694633452.49957336
TTCTCGG200.00694633452.49957362
TAATACG404.5853252E-543.749644
GTAATAC400.002416152334.9997143
GCATAGG450.00429465631.1226881
GGCGCTA450.00430265631.11085718
TCTGGCG604.974213E-429.1664311
CTCTAGG755.5578304E-528.0104181
ACCATAG500.00720071327.99977153
ACTGTTA500.00720071327.99977111
GTCTTCA500.00720071327.99977141
GAGATGC500.00720071327.99977169
Produced by FastQC (version 0.11.5)