FastQCFastQC Report
Wed 25 May 2016
SRR1781031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263115
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT5057319.220873002299374No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG63572.4160538167721337No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC30541.1607091955988826No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCGT22800.8665412462231344No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC13650.518784561883587No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT9010.3424358170381772TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC7350.27934553332193146No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCCTGTATCTCGTATGCCGT6960.2645231172681147No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGACGT6530.2481804534139065No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTAAGCCGT5770.21929574520646866No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTAGTATGCCG4180.158865895140908No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG3880.147464036637972No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT3760.1429032932367976No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC3650.13872261178572107No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC3110.1181992664804363No Hit
CATATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT3070.11667901868004484No Hit
TTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTC3040.11553883282975125No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG2740.10413697432681526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA150.00222320869.9982927
CGAGTAT150.00222320869.9982940
GACGTAT353.6998244E-969.9982946
ACGTATT301.0213989E-769.9982947
ATACGTC150.00222320869.9982945
GCCGTTG150.00222320869.9982938
AGGGGGG52600.064.808370
TACCACG252.3879267E-455.9986318
TCTGCTT60700.053.62307453
CGTCTTC62800.053.6133448
GCCGTCT70050.053.56043246
CTGCTTG60550.053.52470454
CCGTCTT70350.053.4812847
GTCTTCT60150.053.41515449
CTTGAAA74400.053.2513957
AAGCTTG2500.053.19869654
TTGAAAA76250.053.106958
TGCCGTC74700.053.0843845
ATGCCGT78550.053.06681444
TATGCCG78750.053.06537243