FastQCFastQC Report
Wed 25 May 2016
SRR1781030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425876
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7532117.686133992054025No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGT150.00222402969.999295
ACCGACG200.00694932352.49947467
CGCTATG200.00694932352.49947442
GCGTGTG200.00694932352.4994748
CGAGGGG451.683391E-646.666225
TCGTTGT350.001254941239.99959634
CGAGTTC701.9235813E-839.99959660
GCGAACT350.001254941239.99959628
CTAACGC350.001254941239.99959666
GCCGAGT701.9235813E-839.99959658
TCTAACG350.001254941239.99959665
CCGAGTT701.9235813E-839.99959659
GCGACTC400.002417848234.9996543
ACGTTCT400.002417848234.9996539
TTGACGT400.002417848234.9996551
CGACTCC400.002417848234.9996544
TCGGTGG400.002417848234.9996545
CGGTGTA751.4890338E-632.66633634
GCGGCGT652.1010748E-532.3073720
CCTGCGG652.1010748E-532.3073717
Produced by FastQC (version 0.11.5)