FastQCFastQC Report
Wed 25 May 2016
SRR1781030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425876
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4740411.130939522302267No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG64711.5194563675811739No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC32890.7722905258807728No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT16830.39518545304266967No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC14220.33390000845316475No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC10290.24161962637011714RNA PCR Primer, Index 19 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT5510.1293803830222882No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC5370.12609304116691242No Hit
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT5310.1246841803717514RNA PCR Primer, Index 19 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC4430.1040208887093896No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG4340.10190759751664805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC501.4551915E-1162.99809645
AGGGGGG57650.062.53063670
CATCTCG401.0652002E-861.2481536
ACGTATG650.059.22897740
ACGCGTA1450.055.51556427
CGCGTAA1500.053.66504728
ACGCGAA407.474682E-752.49841727
TGACGAC200.006949876452.49841745
GCCGACT800.052.49841746
AACTCGA407.474682E-752.49841737
TGCCGAC800.052.49841745
AAGCCGT608.367351E-1152.4984144
CCGACTT608.367351E-1152.4984147
GACGCGA2700.050.55402826
GCCGTCT79650.050.26841446
CCGTCTT79200.050.24469847
TATGCCG80450.050.16007643
CGTCTTC79600.050.12411548
TTTCGAC352.0856145E-549.9984928
ATTTGCG352.0856145E-549.9984931