Basic Statistics
Measure | Value |
---|---|
Filename | SRR1781030_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 425876 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT | 47404 | 11.130939522302267 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG | 6471 | 1.5194563675811739 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC | 3289 | 0.7722905258807728 | No Hit |
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT | 1683 | 0.39518545304266967 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC | 1422 | 0.33390000845316475 | No Hit |
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC | 1029 | 0.24161962637011714 | RNA PCR Primer, Index 19 (95% over 22bp) |
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT | 551 | 0.1293803830222882 | No Hit |
CTTTACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC | 537 | 0.12609304116691242 | No Hit |
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT | 531 | 0.1246841803717514 | RNA PCR Primer, Index 19 (95% over 21bp) |
TCTTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC | 443 | 0.1040208887093896 | No Hit |
CTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG | 434 | 0.10190759751664805 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGCCGTC | 50 | 1.4551915E-11 | 62.998096 | 45 |
AGGGGGG | 5765 | 0.0 | 62.530636 | 70 |
CATCTCG | 40 | 1.0652002E-8 | 61.24815 | 36 |
ACGTATG | 65 | 0.0 | 59.228977 | 40 |
ACGCGTA | 145 | 0.0 | 55.515564 | 27 |
CGCGTAA | 150 | 0.0 | 53.665047 | 28 |
ACGCGAA | 40 | 7.474682E-7 | 52.498417 | 27 |
TGACGAC | 20 | 0.0069498764 | 52.498417 | 45 |
GCCGACT | 80 | 0.0 | 52.498417 | 46 |
AACTCGA | 40 | 7.474682E-7 | 52.498417 | 37 |
TGCCGAC | 80 | 0.0 | 52.498417 | 45 |
AAGCCGT | 60 | 8.367351E-11 | 52.49841 | 44 |
CCGACTT | 60 | 8.367351E-11 | 52.49841 | 47 |
GACGCGA | 270 | 0.0 | 50.554028 | 26 |
GCCGTCT | 7965 | 0.0 | 50.268414 | 46 |
CCGTCTT | 7920 | 0.0 | 50.244698 | 47 |
TATGCCG | 8045 | 0.0 | 50.160076 | 43 |
CGTCTTC | 7960 | 0.0 | 50.124115 | 48 |
TTTCGAC | 35 | 2.0856145E-5 | 49.99849 | 28 |
ATTTGCG | 35 | 2.0856145E-5 | 49.99849 | 31 |