FastQCFastQC Report
Wed 25 May 2016
SRR1781029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479806
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9086218.93723713334139No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCGA252.389769E-455.99933221
TGCGCGT252.389769E-455.9993325
CGTGCGA200.00694990852.4993810
ACCGCGC200.00694990852.4993810
CGGTGCG200.00694990852.4993816
AGTTCGC200.00694990852.4993857
TCCCGGT200.00694990852.4993823
GTGCGCG305.876305E-446.6661154
CGCGTCT451.683864E-646.666117
GCGCGTC551.3106E-744.5449266
TGCCGGC503.4769182E-641.999519
CTCGCTA350.001255111439.99952313
CTGCCGG654.862686E-737.6918618
TGTGCGC400.002418174434.9995843
AACCTCG501.709121E-434.9995819
TCCGTAT552.9910836E-431.81780413
TAGTCCG552.9910836E-431.8178045
CCGTATT450.00430623631.1107414
ATCCCCG450.00430623631.1107410
GGTGCCG450.00430623631.1107417
Produced by FastQC (version 0.11.5)