FastQCFastQC Report
Wed 25 May 2016
SRR1781029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479806
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT5875812.246199505633527No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG77031.6054405322151035No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC32390.6750645052375336No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC17170.357852965573586No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT16790.34993309796042565No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC11500.23968020408248336No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT10310.21487851339916547No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT6780.14130711162428147No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT6410.13359566157988853No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG5470.11400441011575511No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC5110.10650137764012955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA150.002224270369.9987411
AGGGGGG73300.064.4599770
AACCGAG308.373572E-658.33228712
CGTCTTA601.8189894E-1258.33228748
CGTCTAC653.6379788E-1253.84519248
CCGTCTT96750.053.39439447
TATGCCG98300.053.33577343
GCCGTCT97600.053.2879846
CGTCTTC98250.053.25604648
ATGCCGT98700.053.22599844
GTATGCC98500.053.1564142
TGCCGTC98650.053.1465445
CTCGTAT100200.053.12779239
CGTATGC99950.053.12061341
TCGTATG101800.053.08353440
ATCTCGT99300.053.04537637
AATCTCG100300.053.00503536
TCTCGTA99800.052.99003638
TACGCTG200.006950071552.4990667
TCGTATC200.006950071552.4990640