FastQCFastQC Report
Wed 25 May 2016
SRR1781028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198527
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8733643.99200108801322No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACGA150.002222186470.022
CCGTACC150.002222186470.068
CGTACCT150.002222186470.069
TGGTCGC453.8198777E-1062.22222549
TCGCCGT308.354684E-658.33333252
GTCGCCG452.6884663E-854.44444751
TACCCGC200.006943609652.570
TTATACG200.006943609652.52
CTACCCG200.006943609652.569
TACAGGG200.006943609652.55
CGGTGGT506.166738E-849.00000446
TGCCGGC451.6786998E-646.66666819
TGTACAG305.867591E-446.6666645
TCTGGCG305.867591E-446.66666411
CTCGGTG659.95351E-943.07692344
TCGGTGG659.95351E-943.07692345
AGTGCCT350.001253260840.00000453
CTTTGAT350.001253260840.0000041
CCCTTTT350.001253260840.00000416
TTGGGCG350.001253260840.0000042
Produced by FastQC (version 0.11.5)