FastQCFastQC Report
Wed 25 May 2016
SRR1781028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198527
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT5599228.203720400751536No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG73533.7037783273811624No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC31351.5791302946198755No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC15300.7706760289532406No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT10490.5283916041646728TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC8550.43067189853269333No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTAGTATGCCGT6230.31381121963259406No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCACGTATGCCGT5500.27704040256489043No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGACGT5110.2573957194739255No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4880.2458103935484846No Hit
CTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG4740.23875845602865103No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTAAGCCGT4130.20803215683509046No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC3920.1974542505553401No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3910.19695054073249485No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC3490.1757947281729941No Hit
CTTATACACATCTCCGAGCCCACGAGACCTAGGGTCATCTCGTATGCCGT3240.16320198260186272No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2930.1475869780936598No Hit
CTTATACACATCTCCGAGCCAACGAGACCTTGGGTCATCTCGTATGCCGT2880.14506842897943353No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCAGT2650.13348310305399266No Hit
CTTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2640.1329793932311474No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGGGTCATCTCGTATGCCGT2610.13146826376261164No Hit
CCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG2410.12139406730570654No Hit
TATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCT2340.11786809854578975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA150.002222820469.99496530
TGACGAA150.002222820469.99496545
TACGAGA150.002222820469.99496513
AGGGGGG58050.066.19678570
CCGACTT401.0617441E-861.2455947
TTAAACA2850.058.972842
CACGTAT1900.058.94312739
ATCTCGA1250.058.79576537
ATCACGT2150.058.60043337
AACTCGA308.358247E-658.3291437
TCGAAAG308.358247E-658.3291440
AAAGCCG308.358247E-658.3291443
CGTAATC900.058.32913248
CTTAAAC2900.057.9414631
TCACGTA2200.057.26860438
TCTCGAA1350.057.03293238
CGAATGC1050.056.6625941
ACGTATG2050.056.33740640
CATCACG2550.056.2704636
TCTTTAT2750.056.010081
Produced by FastQC (version 0.11.5)