FastQCFastQC Report
Wed 25 May 2016
SRR1781027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221580
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10436447.09991876523152No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2360.10650780756385955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTG200.006944845452.49966418
GCATTGG200.006944845452.49966433
GTACTTA200.006944845452.49966448
GATTCAG200.006944845452.49966458
TAAGCCG200.006944845452.49966424
TACACTG200.006944845452.4996645
CGGACTT200.006944845452.4996645
ACTTATG352.0820507E-549.9996816
TAAACTC404.5829205E-543.7497252
CTGTAGC350.001250834940.0178071
CGTATCA350.00125361639.99974456
CGTTACA350.00125361639.99974414
AGCGGAT350.00125361639.99974451
TCAGACC350.00125361639.99974457
AAGCATT400.002415310634.99977535
CGGATGA400.002415310634.99977553
TGTTCGT400.002415310634.99977510
TTGTTCG400.002415310634.9997759
TCTGGCG601.2121809E-534.99977511
TGATATA552.985829E-431.81797845
Produced by FastQC (version 0.11.5)