FastQCFastQC Report
Wed 25 May 2016
SRR1781027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221580
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT7065931.888708367181152No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG92184.16012275476126No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC42161.9026987995306437No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC19640.8863615849805939No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT16260.7338207419442188RNA PCR Primer, Index 19 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC11360.5126816499684087No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT8510.38405993320696813No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT7880.35562776423864967No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG5860.26446430183229536No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC5040.22745735174654752RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACTTATGAAAATCTCGTATGCCGT4710.21256431085838073No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC4520.20398952974095136No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT4270.19270692300749165No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC4240.19135301019947648No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3930.17736257784998646No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT3920.1769112735806481No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCAGT3840.17330083942594096No Hit
CTTATACACATCTCCGAGCCCACGAGACATATGAAAATCTCGTATGCCGT3450.15569997292174384No Hit
CTTATACACATCTCCGAGACCACGAGACTTATGAAAATCTCGTATGCCGT3330.1502843216896832No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAGAAAATCTCGTATGCCGT3180.14351475764960736No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC3160.1426121491109306No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG3130.14125823630291542No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT3000.13539128080151638RNA PCR Primer, Index 19 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG2280.10289737340915245No Hit
CTTATACACATCTCCGAGCACACGAGACTTATGAAAATCTCGTATGCCGT2220.10018954779312213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA252.8307113E-670.01128411
ATAACCG252.8307113E-670.01128410
ATAGATG150.002223096669.9954826
ATCCCAC207.917047E-569.9954817
TCCCACG207.917047E-569.9954818
AAAGCGC150.002223096669.9954868
AATCGTC207.917047E-569.9954828
AGGGGGG78450.066.0696770
AAACGAG950.062.6275420
AACTCGT900.062.2182137
GCAAACG750.060.66275418
TCCGAGA2150.060.24227513
TAACGTA352.9662624E-759.9961338
CGAGAAC352.9662624E-759.9961315
ACCACGA2050.059.7522519
AAACTCG1300.059.2269536
AACTCCG1250.058.8094810
ACTCCGA1250.058.8094811
ACGGGGG308.361072E-658.3295770
AACACAT5250.058.0224535
Produced by FastQC (version 0.11.5)