FastQCFastQC Report
Wed 25 May 2016
SRR1781026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239939
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4225117.60905896915466No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG19600.8168742888817575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGGC252.3874929E-455.99866517
GCGTGCT252.3874929E-455.99866538
ATCATGG252.3874929E-455.99866516
GCCGTAT2650.055.47037554
TCGCCGT2600.055.1909952
TGGTCGC2600.055.1909949
CGTATCA2850.052.8057656
GATCACT200.006946020852.4987525
CCTCGCA200.006946020852.4987512
CCTCCAG200.006946020852.4987516
CAATGTG200.006946020852.498753
TCAACCG200.006946020852.4987517
GTAGATC2950.052.2021538
TCGGTGG3000.051.33210845
ATCTCGG2950.051.01573642
GTATCAT2950.051.01573657
AGATCTC3000.050.1654740
CTCGGTG3000.050.1654744
TAGATCT3000.050.1654739
GATCTCG3000.050.1654741
Produced by FastQC (version 0.11.5)