FastQCFastQC Report
Wed 25 May 2016
SRR1781026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239939
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT146486.104884991602033No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT72483.0207677784770297No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30711.279908643446876No Hit
CATAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT30531.2724067367122478No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG21930.9139823038355582No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT11880.49512584448547337No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT9040.37676242711689223No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT6790.2829885929340374No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC4810.20046761885312517No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC4560.19004830394391908No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3300.13753495680152036No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT2700.11252860101942576RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2610.10877764765211156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC150.00222275370.0000118
CGAGTAT150.00222275370.0000141
ATCGTAA252.834142E-670.0000139
GTGTATC150.00222275370.0000115
CATCGAT150.00222275370.000011
GCCGTAT252.834142E-670.0000146
CGTAAGA207.915854E-570.041
AGGGGGG37900.064.274470
CATATAC19650.061.8066141
GCCGACT800.061.2546
ATATACA20150.060.6203462
ACTTACG352.9659896E-760.00000427
CTTTCGA700.060.00000428
TGCCGAC950.058.94737245
TTCGTAA308.359979E-658.33333630
TGCCGTA308.359979E-658.33333645
TATGCCG42100.057.61282743
CCGTCTT40350.057.50929647
GCCGTCT41150.057.41190746
ATGCCGT41850.057.28793344
Produced by FastQC (version 0.11.5)