FastQCFastQC Report
Wed 25 May 2016
SRR1781025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208178
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3413916.39894705492415No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG17920.8608018138323934No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA150.002225274569.976761
TGTACGT252.3904038E-455.98135812
TGGCGGC200.00693342952.5203719
TGATCTC200.00693342952.5203728
TGGTCGC2800.049.98335649
AGATCTC2900.049.49006340
GATCTCG2800.048.75719541
CGGTGGT2950.048.62787646
TCGCCGT2750.048.35915452
GCCGTAT2800.047.49559854
GTAGATC3050.047.0674438
TCGGTGG3050.047.0335245
TAGATCT3100.046.29715339
ATCTCGG2950.046.266942
CTCGGTG3050.045.8973944
TCTCGGT3050.045.8973943
CGTATCA2950.045.08056656
GTCGCCG3000.044.32922751
CGCCGTA3050.043.60251653
GGTGGTC3350.042.82156447
Produced by FastQC (version 0.11.5)