FastQCFastQC Report
Wed 25 May 2016
SRR1781025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208178
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT110155.291145077769985No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT62112.9835045009559127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27651.328190298686701No Hit
CATAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT24981.1999346712909147No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG16670.8007570444523437No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT10790.5183064492885896No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT7760.3727579283113489No Hit
CAAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT5840.2805291625435925No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4900.2353754959697951No Hit
CACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4340.20847543928753282No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3000.14410744651211946No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2170.10423771964376641No Hit
ACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2130.10231628702360479No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGGA150.002221037470.0103331
GGACGTC150.002223155269.99351535
TGCCGAC650.064.6249245
AGGGGGG31700.064.0319270
ATCGTAT453.8198777E-1062.23140739
GCGCACG1100.060.4634729
CGACTTC700.060.00885848
CATATAC15200.059.6469651
GCCGACT650.059.23951346
CGCACGT950.058.95607430
CGAATCT950.058.9419134
ATATACA15700.058.19332
CCGACTT553.6379788E-1157.2811847
CGTCTTC35150.057.06389648
CCGTCTT34500.057.02290747
TCTTCTG34500.057.00921250
GCCGTCT35000.056.908446
CTTCTGC34850.056.73792651
GTCTTCT34800.056.7325149
CTTGAAA36150.056.73056457
Produced by FastQC (version 0.11.5)