FastQCFastQC Report
Wed 25 May 2016
SRR1781024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158521
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3242120.452179837371702No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG15310.9658026381362721No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTAG150.00221968970.0132523
TTTCCCG150.00221968970.0132550
GTATCCG207.9001635E-570.013251
CGTTTAT150.00221968970.0132521
TGGTTCC150.002222469569.9911713
TCCGACA207.912525E-569.9911654
CATCTGG353.6907295E-969.9911659
GACATCT308.35319E-658.3259747
AATGCAT252.3825E-456.010638
TATCCGA252.3825E-456.01062
TCGTTTA200.006935842352.50993720
GCTCTCT200.006935842352.5099371
GTTGTGC200.006935842352.5099371
CGCGTCT200.006944505552.4933747
GGTTCCT200.006944505552.49337414
TTACAGC200.006944505552.49337464
TGTCGGT200.006944505552.49337443
CTCAGTG200.006944505552.49337428
ATCTGGC200.006944505552.49337410
ACTTCAG352.0769327E-550.00946421
Produced by FastQC (version 0.11.5)