FastQCFastQC Report
Wed 25 May 2016
SRR1781024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158521
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT106076.6912270298572425No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT56473.562304048044108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24851.5676156471382339No Hit
CATAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT22831.4401877353789088No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG15720.9916667192359372No Hit
CAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT9410.593612202799629No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT7120.44915184738930486No Hit
CAAAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT5820.36714378536597675No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC3790.2390850423603182No Hit
CACTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC3550.22394509244831914No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT3100.19555768636332094No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2860.1804177364513219RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC2340.14761451164199066No Hit
CTAAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1810.1141804555863261No Hit
AAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT1700.10724131187665988RNA PCR Primer, Index 47 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1700.10724131187665988No Hit
ACTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1670.10534881813765999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA301.01703336E-770.01546546
ATAAACG207.898928E-570.01546538
TGCAGAC150.002219411570.01546545
ACGACTA150.002219411570.01546547
GCGAAAA150.002222191569.9933856
CGCGAAA150.002222191569.9933855
AGGGGGG28750.063.42008670
CCGACTT800.061.2635347
CATATAC15100.060.4909671
ATATACA15400.059.767072
ATATCGT950.058.96039237
CGACTTC950.058.96039248
ATCTCGA900.058.3462237
AACTCGT601.8189894E-1258.3462237
TAAGACG308.336014E-658.3462243
CGTCTAC553.45608E-1157.28537848
GCCGACT750.056.0123746
ATCTCGT33250.055.27536437
GATCTCG34100.055.1294836
AAGCCGT700.055.01214644
Produced by FastQC (version 0.11.5)