FastQCFastQC Report
Wed 25 May 2016
SRR1781023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43639
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3034169.52725772817892No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG18294.191205114690987No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT2000.45830564403400625No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC1070.24519351955819335No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG990.22686129379683312No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG710.16269850363207222No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG570.1306171085496918No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG540.12374252388918171No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG490.11228488278833154No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG470.10770182634799147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGG207.815437E-570.0505218
TGCCGGG150.002204204470.05051419
TGCCGGC150.002204204470.05051419
GCGCTGC150.002204204470.05051415
GGGGTGC150.002204204470.05051415
GGGTGTC150.002204204470.05051440
GTGCGCG150.002214275369.9701844
TGTGCGC150.002214275369.9701843
GGTGTCG150.002214275369.97018441
AAAAAGG1950.055.68118370
GGAGTGG200.00688775552.5378933
GTGTCGG200.006919133552.4776442
CTGGGGC200.006919133552.4776412
CGCGTCT200.006919133552.477647
CGTCTGG352.0594793E-549.9787039
TCGCCGT3350.048.0392352
GTGTAGA3300.047.7617236
CTGGTGT3450.047.7155733
GGTGTAG3450.047.7155735
GTGGTCG3300.047.70694448
Produced by FastQC (version 0.11.5)