FastQCFastQC Report
Wed 25 May 2016
SRR1781023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781023_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43639
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1013723.229221567863608No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT563712.917344577098467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27586.320034831228946No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT21794.993239991750499No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG16883.8680996356470128No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT9502.1769518091615296No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT6601.5124086253122206No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT5201.1915946744884163No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2740.6278787323265886No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2740.6278787323265886No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2660.6095465065652284No Hit
ACTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2090.4789293980155366No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1810.4147666078507757Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1750.4010174385297555No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1570.35976993056669493No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT1220.27956644286074384No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1130.25894268887921357No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1110.2543596324388735No Hit
AAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1090.24977657599853342Illumina PCR Primer Index 9 (95% over 22bp)
TCTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1050.2406104631178533No Hit
CAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC970.22227823735649302No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT860.19707142693462268No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT820.18790531405394256No Hit
ACTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG800.18332225761360252No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC780.17873920117326245No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT770.1764476729530924No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC700.16040697541190221No Hit
CACTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC700.16040697541190221No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT680.15582391897156214Illumina PCR Primer Index 9 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG670.1535323907513921No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT650.14894933431105203No Hit
CATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG640.14665780609088203No Hit
CATATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT620.14207474965054195No Hit
CATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC600.13749169321020188No Hit
CACATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC590.13520016499003185No Hit
CATAAACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT540.12374252388918171No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT530.12145099566901166No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG520.11915946744884164No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG520.11915946744884164No Hit
CATAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG500.11457641100850156No Hit
CATATACACATCTCCGAGCCCACGAGACGTAAACAGATCTCGTATGCCGT490.11228488278833154No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGA490.11228488278833154No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT480.1099933545681615No Hit
CATATACACATCACCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT440.10082724168748139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATG207.847969E-569.9919840
ACTGATT207.847969E-569.9919853
TACTGAT207.847969E-569.9919852
CTACTGA207.847969E-569.9919851
CAAGAGA301.0021358E-769.99197421
AGTGGGG150.002211542369.99197470
CAACACA150.002211542369.9919744
AGTATGA150.002211542369.99197441
CAATACA455.456968E-1269.9919741
CCCAAGA150.002211542369.99197419
AAGATCT252.7972928E-669.99197434
TAACATA150.002211542369.99197431
CACTTAT301.0021358E-769.9919741
AGAGACG301.0021358E-769.99197423
CGAGAAG301.0021358E-769.99197423
CCAAGAG252.7972928E-669.99197420
CAAGTAT150.002211542369.99197439
AACATAT150.002211542369.99197432
GAAGTTA353.607056E-969.9919726
CTGCATG750.069.9919754
Produced by FastQC (version 0.11.5)