FastQCFastQC Report
Wed 25 May 2016
SRR1781022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46108
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3482675.53136115207772No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG20674.482953066712935No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT2180.4728029842977357No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG1320.2862843758133079No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC1230.26676498655330966No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG770.1669992192244296No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG660.14314218790665395No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480.10410340938665742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT301.003591E-769.990241
CTTTATA352.916113E-759.991632
TTTATAC352.916113E-759.991633
AAAAAGG2150.058.66010370
GCTGGTG3500.052.99260732
TGCTGGT3500.052.99260731
CTGGTGT3500.052.99260733
CGTTGCT3500.052.99260728
TGGTGTA3500.052.99260734
GTTGCTG3500.052.99260729
TTGCTGG3500.052.99260730
GAAGAAG200.006913804452.49268368
GTAGATC3600.052.4926838
GTGTAGA3600.052.4926836
GTATCAT3600.052.4926857
CATTAAA3600.052.4926861
TCTCGGT3600.052.4926843
CGTATCA3600.052.4926856
TATCATT3600.052.4926858
TGTAGAT3600.052.4926837