FastQCFastQC Report
Wed 25 May 2016
SRR1781022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781022_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46108
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1237426.836991411468723No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT613813.312223475318815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29996.504294265637199No Hit
CATAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT26465.73870044243949No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG18864.090396460484081No Hit
CAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT10222.2165350915242477No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT7991.7328880020820683No Hit
CAAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT5881.2752667649865534No Hit
CACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3570.7742691073132645No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3190.6918539082154941No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2600.563893467511061No Hit
ACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2180.4728029842977357No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2060.4467771319510714No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT1770.38388132211329923RNA PCR Primer, Index 42 (95% over 22bp)
CTAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1700.36869957491107835No Hit
AAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT1400.30363494404441743RNA PCR Primer, Index 42 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1300.2819467337555305RNA PCR Primer, Index 32 (95% over 21bp)
CAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1240.2689338075821983No Hit
TCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1170.25375206037997744No Hit
CACTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1120.24290795523553396No Hit
TCTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1110.24073913420664525No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT1090.23640149214886785No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1010.2190509239177583No Hit
ACTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG950.2060379977444261No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC800.17350568231109567No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG780.16916804025331827No Hit
CATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG770.1669992192244296No Hit
CATTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC760.1648303981955409RNA PCR Primer, Index 32 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT740.1604927561377635RNA PCR Primer, Index 42 (95% over 21bp)
CACATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC730.1583239351088748No Hit
CATAAACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT700.15181747202220872No Hit
CATAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG650.14097336687776524No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT650.14097336687776524No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT630.13663572481998784No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC610.13229808276221047No Hit
CATATACACATCACCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT590.12796044070443308No Hit
CATAAACACATCACCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT550.11928515658887828No Hit
CATAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT480.10410340938665742No Hit
CATATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT480.10410340938665742No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG480.10410340938665742No Hit
CATAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT470.10193458835776872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCACG207.810329E-570.0684118
ATCCGAG150.002202780770.06840512
CACAGAG150.002202780770.06840512
ACTCGTA252.7997467E-669.9924138
GAGAAAT252.7997467E-669.9924124
CGATAAC150.002212296769.9924135
TATACCC150.002212296769.992413
AAACAGG150.002212296769.9924166
ATGACGA207.85251E-569.9924144
ACGTCAT207.85251E-569.9924147
ACCACGA207.85251E-569.9924119
TAAGCCG301.00337274E-769.9924143
TAACGTC150.002212296769.9924145
CGCTCTC353.612513E-969.9924135
CGCTCTA150.002212296769.9924135
GAAGTCT150.002212296769.9924146
CTCTCGT301.00337274E-769.9924137
CTCTCGA150.002212296769.9924137
TCGAACT401.3096724E-1069.9924134
TTCTGAT150.002212296769.9924152
Produced by FastQC (version 0.11.5)