FastQCFastQC Report
Wed 25 May 2016
SRR1781021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65541
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4892274.64335301566958No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG24203.692345249538457No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT2760.4211104499473612No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC1720.26243114996719613No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1540.23496742497062909No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG1400.21360674997329918No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1030.1571535374803558No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG910.13884438748264444No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.11290642498588671No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.10375184998703102No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG660.10070032498741247No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTG150.002215523570.08
TACTGGT150.002215523570.031
AGTTGCT150.002215523570.028
CGTGTGG207.873018E-570.09
TGTGGCG150.002215523570.011
ACTGGTG150.002215523570.032
CCGGCGG401.3278623E-1070.021
TGCCGGC401.0464646E-861.2519
GCCGGCG553.45608E-1157.2727320
TCTGGCG452.651359E-854.44444711
TAGTGTA200.006922950552.534
GGCGTAG200.006922950552.51
TATAAAC200.006922950552.53
CTGGCGG200.006922950552.512
CTGCCGG407.375547E-752.518
TGGCGCG200.006922950552.513
GTGTGGC200.006922950552.510
CCGACGG200.006922950552.521
AAAAAGG2250.051.33333670
ATCATTA3500.048.059
Produced by FastQC (version 0.11.5)