##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781021_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65541 Sequences flagged as poor quality 0 Sequence length 76 %GC 92 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.061625547367296 32.0 27.0 32.0 13.0 32.0 2 28.707404525411576 32.0 32.0 32.0 13.0 32.0 3 28.674142902915733 32.0 27.0 32.0 13.0 32.0 4 28.054515494118185 32.0 27.0 32.0 13.0 32.0 5 28.86919638089135 32.0 32.0 32.0 13.0 32.0 6 32.91745624876032 37.0 37.0 37.0 13.0 37.0 7 32.52181077493477 37.0 32.0 37.0 13.0 37.0 8 32.922369204009705 37.0 37.0 37.0 13.0 37.0 9 33.057368669992826 37.0 37.0 37.0 13.0 37.0 10-11 32.90306067957462 37.0 34.5 37.0 13.0 37.0 12-13 32.67254848110343 37.0 32.0 37.0 13.0 37.0 14-15 33.29048229352619 37.0 34.5 37.0 17.5 37.0 16-17 33.50492821287438 37.0 37.0 37.0 17.5 37.0 18-19 33.123022230359624 37.0 34.5 37.0 13.0 37.0 20-21 33.48034055018996 37.0 37.0 37.0 17.5 37.0 22-23 33.558589279992674 37.0 37.0 37.0 17.5 37.0 24-25 33.163302360354585 37.0 37.0 37.0 13.0 37.0 26-27 33.61803298698524 37.0 37.0 37.0 20.0 37.0 28-29 33.63715079110786 37.0 37.0 37.0 22.0 37.0 30-31 33.42598526113425 37.0 37.0 37.0 13.0 37.0 32-33 33.57322134236585 37.0 37.0 37.0 13.0 37.0 34-35 33.64290291573214 37.0 37.0 37.0 22.0 37.0 36-37 34.16932912222883 37.0 37.0 37.0 24.5 37.0 38-39 34.250278451656214 37.0 37.0 37.0 27.0 37.0 40-41 33.777131871652855 37.0 37.0 37.0 20.0 37.0 42-43 33.746845486031646 37.0 37.0 37.0 22.0 37.0 44-45 33.57272546955341 37.0 37.0 37.0 22.0 37.0 46-47 33.80528981858684 37.0 37.0 37.0 20.0 37.0 48-49 34.20370455134953 37.0 37.0 37.0 24.5 37.0 50-51 34.469171968691356 37.0 37.0 37.0 27.0 37.0 52-53 33.77811598846524 37.0 37.0 37.0 22.0 37.0 54-55 33.89416548420073 37.0 37.0 37.0 24.5 37.0 56-57 33.79866800933767 37.0 37.0 37.0 20.0 37.0 58-59 33.52351200012206 37.0 37.0 37.0 17.5 37.0 60-61 33.371988526266 37.0 37.0 37.0 17.5 37.0 62-63 33.01296898124838 37.0 37.0 37.0 13.0 37.0 64-65 32.85767687401779 37.0 34.5 37.0 13.0 37.0 66-67 32.96615095894173 37.0 37.0 37.0 13.0 37.0 68-69 33.20571855784928 37.0 37.0 37.0 13.0 37.0 70-71 33.38450740757693 37.0 37.0 37.0 13.0 37.0 72-73 33.6175828870478 37.0 37.0 37.0 17.5 37.0 74-75 33.562586777742176 37.0 37.0 37.0 17.5 37.0 76 34.11144169298607 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.4656838694979157 11101 2 -0.06153299900863374 11101 3 -0.7608207217125234 11101 4 -0.4130148880520004 11101 5 -1.1600117528856195 11101 6 0.34792196963062594 11101 7 0.8197505550916659 11101 8 -1.5111302197677823 11101 9 -0.2905248268915628 11101 10-11 -0.5664142726402304 11101 12-13 -0.7847019300079445 11101 14-15 -0.7753307455697325 11101 16-17 -0.5836242091667714 11101 18-19 0.6086419019906089 11101 20-21 0.5077163603275068 11101 22-23 -0.8974219279608278 11101 24-25 -1.3758064934209813 11101 26-27 -1.3541023875142812 11101 28-29 -0.9196637686102775 11101 30-31 -0.9583184674627105 11101 32-33 -0.7402936437548675 11101 34-35 0.5720380340803857 11101 36-37 -0.011405459164663512 11101 38-39 -0.03592207350039445 11101 40-41 -1.2250276936250444 11101 42-43 -0.26959588908725607 11101 44-45 -0.511925843275975 11101 46-47 -0.5640713648029418 11101 48-49 0.07705037532571168 11101 50-51 -1.516282077305057 11101 52-53 -0.9484921508006394 11101 54-55 -0.6735686075399627 11101 56-57 -0.3715494125055798 11101 58-59 0.36553307765826304 11101 60-61 -0.28847999638334443 11101 62-63 -1.2381559279463517 11101 64-65 1.142394035989632 11101 66-67 0.4937294012588893 11101 68-69 -0.09212052477204224 11101 70-71 0.9350319324171181 11101 72-73 0.20041375381361348 11101 74-75 -0.5508504622030159 11101 76 0.041881437138471256 11102 1 -1.433799811526903 11102 2 -1.4243349313757854 11102 3 -0.14874342702653465 11102 4 1.1386759332040377 11102 5 -0.305905472692384 11102 6 1.3846369454760392 11102 7 0.20235925074383943 11102 8 0.11109200245444129 11102 9 -0.19825429549059237 11102 10-11 -0.5296992967948171 11102 12-13 -0.889774393776058 11102 14-15 -1.0378911320431712 11102 16-17 -0.11381744588174314 11102 18-19 -0.5261407067050499 11102 20-21 -0.938418905372977 11102 22-23 0.2083751734884487 11102 24-25 0.452937467931676 11102 26-27 -0.3717352377558285 11102 28-29 -0.6853642517020688 11102 30-31 -0.06508175248686854 11102 32-33 -0.16203277418964745 11102 34-35 -1.2830344296877243 11102 36-37 -0.058990000227467476 11102 38-39 -0.9649075807467753 11102 40-41 -1.7916943602917144 11102 42-43 0.3050417920721671 11102 44-45 0.5849340601056667 11102 46-47 -1.711172814078303 11102 48-49 -1.6792298179110077 11102 50-51 -0.6793255555659314 11102 52-53 0.6722807960592618 11102 54-55 -0.21076667517281322 11102 56-57 -1.7961870936649973 11102 58-59 -0.24147175325961 11102 60-61 -0.398383378025855 11102 62-63 -0.03767283615891159 11102 64-65 -0.7288620026577064 11102 66-67 -0.8202802605768582 11102 68-69 0.13565725300573206 11102 70-71 0.0461430435282324 11102 72-73 -0.5469292413554712 11102 74-75 -0.1725895926377916 11102 76 -0.09241807976974314 11103 1 -0.37901720283124973 11103 2 0.36957811210247726 11103 3 0.014734833843032646 11103 4 0.6847628897257749 11103 5 0.3488771360032672 11103 6 0.8168108585195171 11103 7 0.23975055509166765 11103 8 0.5110920024544399 11103 9 0.6539196175528872 11103 10-11 -0.3075253837513401 11103 12-13 0.23196473665872475 11103 14-15 0.9268914766524858 11103 16-17 0.6074869019443412 11103 18-19 0.5486419019906066 11103 20-21 0.21549413810528506 11103 22-23 -0.03075526129416062 11103 24-25 0.34641572880123306 11103 26-27 0.8547865013746048 11103 28-29 0.39811400916749307 11103 30-31 0.38057042142617803 11103 32-33 0.9797063562451385 11103 34-35 0.5187047007470582 11103 36-37 1.0497056519464465 11103 38-39 -0.03925540683372475 11103 40-41 -0.4716943602917141 11103 42-43 -0.22626255575391951 11103 44-45 0.04362971227957502 11103 46-47 0.04926196853038789 11103 48-49 0.6081614864368206 11103 50-51 0.7237179226949451 11103 52-53 -0.2429365952450766 11103 54-55 0.5953202813489327 11103 56-57 -0.07488274583891297 11103 58-59 -0.526689144563953 11103 60-61 0.26596444806109787 11103 62-63 0.6340662942758684 11103 64-65 0.4246162582118558 11103 66-67 0.6392849568144499 11103 68-69 0.5856572530057349 11103 70-71 0.8661430435282327 11103 72-73 -0.1673640239641614 11103 74-75 0.7791495377969966 11103 76 0.8041036593606918 11104 1 -0.07044577425982368 11104 2 -0.5599456974213339 11104 3 0.0614015005096995 11104 4 -0.2733323483694612 11104 5 1.509829516955648 11104 6 1.6082394299480853 11104 7 0.37213150747261636 11104 8 0.5396634310258719 11104 9 0.5015386651719353 11104 10-11 -0.10371585994181487 11104 12-13 -0.046606691912707277 11104 14-15 -0.8173942376332306 11104 16-17 -0.3891797647223214 11104 18-19 -0.841358098009394 11104 20-21 -2.2764106237994817 11104 22-23 -0.17361240415129942 11104 24-25 -0.32501284262733066 11104 26-27 -0.5342611176730117 11104 28-29 0.4057330567865449 11104 30-31 -0.13133434047858117 11104 32-33 -0.04505554851677118 11104 34-35 0.32203803408038567 11104 36-37 0.2520866043273955 11104 38-39 -0.15020778778610833 11104 40-41 0.5416389730416142 11104 42-43 -0.7291196986110577 11104 44-45 0.3722011408510113 11104 46-47 -0.35454755527913306 11104 48-49 0.002447200722528464 11104 50-51 -1.266282077305057 11104 52-53 0.3332538809453993 11104 54-55 -0.2894416234129764 11104 56-57 -1.238216079172247 11104 58-59 -0.31859390646871333 11104 60-61 -0.9392736471769965 11104 62-63 0.19835200856158508 11104 64-65 0.17175911535471755 11104 66-67 1.0373801949096872 11104 68-69 0.6237524911009729 11104 70-71 0.5818573292425171 11104 72-73 1.1321597855596437 11104 74-75 0.003911442558894862 11104 76 -0.8263725311154957 11105 1 1.479243666733968 11105 2 0.4234911555807379 11105 3 1.2860391816691177 11105 4 0.3995454984214284 11105 5 1.6506162664380497 11105 6 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-0.04854113014207684 11105 68-69 0.02696160083181809 11105 70-71 -0.2147265216891583 11105 72-73 -0.31866837179025254 11105 74-75 -0.22693741872474504 11105 76 1.0597558332737407 11106 1 -0.02282672664077623 11106 2 0.9400543025786661 11106 3 -0.0576461185379209 11106 4 -0.1780942531313663 11106 5 0.24792475505088873 11106 6 0.44157276328142103 11106 7 1.8721315074726164 11106 8 -0.05557466421222301 11106 9 0.26344342707669455 11106 10-11 -0.37752538375134037 11106 12-13 -0.4156543109603277 11106 14-15 -0.2935847138237051 11106 16-17 0.0632011876586347 11106 18-19 0.8848323781810876 11106 20-21 0.4259703285814709 11106 22-23 0.9216256910867884 11106 24-25 0.5797490621345673 11106 26-27 -0.36759445100634025 11106 28-29 0.3581140091674939 11106 30-31 0.03533232618808313 11106 32-33 -0.19981745327867628 11106 34-35 0.6077523197946775 11106 36-37 0.12113422337501589 11106 38-39 0.6950303074519866 11106 40-41 -0.07740864600599906 11106 42-43 0.7946898251984607 11106 44-45 0.0864868551367195 11106 46-47 -0.06883326956484837 11106 48-49 -0.17612422784889503 11106 50-51 0.34086077983779717 11106 52-53 0.8451586428501585 11106 54-55 0.5796059956346369 11106 56-57 0.6189267779706142 11106 58-59 0.9195013316265204 11106 60-61 1.5607263528230035 11106 62-63 0.4007329609425412 11106 64-65 0.24318768678328695 11106 66-67 -0.35547694794745865 11106 68-69 -0.6143427469942679 11106 70-71 0.0461430435282324 11106 72-73 0.5488264522263151 11106 74-75 0.11105429970174896 11106 76 0.6736274688845043 11107 1 -1.3579915618056084 11107 2 0.30086016338452737 11107 3 -0.4257779866697895 11107 4 0.8878398128026994 11107 5 0.15451816164429388 11107 6 0.42142624313490273 11107 7 0.7787249140660251 11107 8 0.8803227716852078 11107 9 1.1077657713990376 11107 10-11 0.6993976931717398 11107 12-13 -0.19495834026435688 11107 14-15 0.3391991689601781 11107 16-17 0.7838971583545984 11107 18-19 0.7932572866059928 11107 20-21 0.3701095227206679 11107 22-23 0.776937046398146 11107 24-25 1.310518292903808 11107 26-27 0.34760701419511975 11107 28-29 -0.9367577857043017 11107 30-31 -0.2540449631892088 11107 32-33 0.5391935357323163 11107 34-35 1.238704700747057 11107 36-37 -1.1233712711304733 11107 38-39 -0.2692554068337287 11107 40-41 0.5929210243236724 11107 42-43 0.47142975193838765 11107 44-45 -0.0948318261819594 11107 46-47 -0.25381495454652736 11107 48-49 0.28816148643682027 11107 50-51 -1.4265384875614657 11107 52-53 1.2993710970626111 11107 54-55 -0.5641668981382537 11107 56-57 -0.13565197660813766 11107 58-59 0.10997752210271017 11107 60-61 -0.010702218605572966 11107 62-63 -0.2595234493138747 11107 64-65 -0.5251273315317349 11107 66-67 -0.15584324831375795 11107 68-69 -0.02459915725067674 11107 70-71 0.4307584281436192 11107 72-73 -0.8440306906308308 11107 74-75 0.028636717284172164 11107 76 0.7102575055145408 11108 1 0.762887559073512 11108 2 1.511482874007239 11108 3 2.013782452890652 11108 4 0.3933343182972031 11108 5 1.8669723740985056 11108 6 0.22728704899570573 11108 7 0.49117912652023676 11108 8 0.6110920024544413 11108 9 0.834871998505264 11108 10-11 1.789141282915331 11108 12-13 0.7986314033253947 11108 14-15 1.0278438576048643 11108 16-17 1.7298678543252919 11108 18-19 1.253879997228701 11108 20-21 1.0450179476290913 11108 22-23 1.552578072039175 11108 24-25 1.8297490621345673 11108 26-27 1.3585960251841271 11108 28-29 1.108114009167494 11108 30-31 0.7972370880928423 11108 32-33 0.8835158800546594 11108 34-35 0.7387047007470571 11108 36-37 1.3473246995654975 11108 38-39 0.766458878880556 11108 40-41 1.8630675444701907 11108 42-43 0.27088030138894226 11108 44-45 0.7888678075176756 11108 46-47 1.0264048256732536 11108 48-49 0.9548281531034917 11108 50-51 2.0670512560282717 11108 52-53 0.15468245237396872 11108 54-55 1.72246313849179 11108 56-57 1.357022016065848 11108 58-59 1.0147394268646153 11108 60-61 0.42977397187061683 11108 62-63 -0.4326003723907945 11108 64-65 -0.05443136083576405 11108 66-67 1.025475433004921 11108 68-69 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30-31 0.896195421426178 21612 32-33 -0.21841864375486608 21612 34-35 -0.042545299252942925 21612 36-37 -0.1534193480535535 21612 38-39 0.5276195931662713 21612 40-41 -1.3229443602917144 21612 42-43 -0.24251255575391895 21612 44-45 0.2561297122795807 21612 46-47 -0.34636303146960756 21612 48-49 -0.44621351356317973 21612 50-51 -0.9850320773050569 21612 52-53 -0.9866865952450823 21612 54-55 -0.3065547186510713 21612 56-57 -0.7017577458389113 21612 58-59 -0.11918914456395413 21612 60-61 -0.8440355519389016 21612 62-63 -0.9534337057241302 21612 64-65 -0.8260087417881437 21612 66-67 -0.9038400431855536 21612 68-69 -0.6924677469942679 21612 70-71 -1.1726069564717676 21612 72-73 -0.3492390239641665 21612 74-75 0.7710245377969898 21612 76 -0.7608963406393059 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 6.0 12 21.0 13 84.0 14 138.0 15 240.0 16 387.0 17 582.0 18 782.0 19 745.0 20 726.0 21 764.0 22 798.0 23 768.0 24 866.0 25 988.0 26 1014.0 27 1145.0 28 1269.0 29 1366.0 30 1668.0 31 1942.0 32 2490.0 33 3326.0 34 4914.0 35 7980.0 36 30529.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 87.47043835156619 1.92703803725912 2.1452220747318473 8.457301536442838 2 84.64813232421875 1.90582275390625 10.26763916015625 3.17840576171875 3 84.60200485192475 2.406127462199234 10.497245998687845 2.494621687188172 4 85.4655864268168 6.987992249126501 4.377412611952823 3.169008712103874 5 84.8583329518927 3.587067637051616 9.857951511267755 1.6966478997879189 6 87.32091362658488 8.330663248958667 2.406127462199234 1.9422956622572132 7 86.04995346424376 3.6603042370424617 2.306952899711631 7.982789399002152 8 86.94710181413161 8.262003936467249 2.13911902473261 2.651775224668528 9 85.39692711432538 2.4839413496895073 1.815657374773043 10.303474161212065 10-11 86.67322744541585 5.219633511847546 5.331777055583528 2.77536198715308 12-13 85.83253230802093 2.554889305930639 5.86808257426649 5.744495811781938 14-15 89.91242123251095 4.334691261958164 2.5785386246776825 3.174348880853206 16-17 89.45621824506797 2.7585785996551775 2.048336155993958 5.736866999282891 18-19 86.12134666880277 2.2879332311049065 5.2128868850082775 6.377833215084033 20-21 89.57483006171944 2.2765244856076965 2.683918612723819 5.464726839949038 22-23 89.48046994202014 2.065913945682026 2.0582850167836435 6.395331095514189 24-25 87.39308576791313 4.835842304845761 1.9540221115036278 5.8170498157374695 26-27 90.06141990615345 4.7403959867241445 2.0997215122267576 3.098462594895662 28-29 89.57215782927518 2.7271409277162673 2.5050933591752957 5.195607883833258 30-31 87.42025579194774 2.59302219101981 7.335704038338268 2.651017978694179 32-33 89.86676230884282 2.48084612801807 3.0363847257409726 4.6160068373981265 34-35 89.81837606837607 2.4244505494505497 5.038156288156288 2.719017094017094 36-37 90.26512202939695 2.155928995527878 5.307782712883679 2.271166262191492 38-39 90.1443922095366 2.17809390072654 5.636791012882349 2.0407228768545087 40-41 89.98145703449907 4.780727525239036 2.9577174601478857 2.280097980114005 42-43 87.72336756649524 4.766446414673972 5.16473119592254 2.3454548229082417 44-45 87.20971039702158 2.223900637797919 5.681436723732796 4.88495224144771 46-47 89.55965150521064 1.9881292055112223 3.1744457498588625 5.277773539419277 48-49 90.45711844494286 1.950687356006164 5.189881143101265 2.402313055949711 50-51 92.64277322591965 2.0147693809981537 3.0133809371233276 2.3290764559588655 52-53 87.62835477029645 2.216170030972979 5.203613005599548 4.951862193131017 54-55 89.78478627392641 2.154425956865707 2.680826066723121 5.379961702484761 56-57 89.64654363465749 2.274235754556482 2.5000572182762806 5.579163392509747 58-59 87.52002563280999 4.770296455653713 5.031201843121099 2.6784760684151907 60-61 86.69077371416365 2.7929082559008864 5.601074136799865 4.915243893135594 62-63 86.90789523874915 5.211361087588401 4.999275246225559 2.8814684274368894 64-65 86.79605132665048 5.512579911810928 5.547672449306541 2.143696312232038 66-67 86.77011336415373 8.573259486428343 2.360354587204956 2.296272562212966 68-69 86.95910894110467 8.799969484284405 1.9896246566981994 2.2512969179127253 70-71 87.21714451378611 8.602774005523598 1.9286815081556983 2.251399972534599 72-73 87.76696806684217 8.053107473961314 1.9983976193201327 2.181526839876388 74-75 89.74869693292734 6.000595251722796 2.044460724833444 2.206247090516419 76 93.21764876455596 2.5136211711917955 2.1488637577644494 2.1198663064877983 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 3.0 17 5.0 18 3.5 19 5.5 20 8.0 21 7.0 22 11.5 23 13.5 24 13.5 25 16.0 26 21.5 27 28.0 28 41.5 29 54.0 30 48.5 31 53.0 32 68.0 33 73.0 34 72.0 35 85.5 36 119.5 37 139.0 38 141.0 39 161.5 40 183.5 41 215.5 42 244.0 43 248.0 44 372.5 45 1048.5 46 1604.0 47 1724.5 48 1121.0 49 375.0 50 353.0 51 306.5 52 259.0 53 247.0 54 236.0 55 235.5 56 233.5 57 224.0 58 216.0 59 219.5 60 233.0 61 229.0 62 215.0 63 188.0 64 148.5 65 125.0 66 120.5 67 127.0 68 108.5 69 101.5 70 93.5 71 74.0 72 77.0 73 74.5 74 69.0 75 69.0 76 63.5 77 73.0 78 87.5 79 87.0 80 83.5 81 89.5 82 101.5 83 104.0 84 117.0 85 139.0 86 146.5 87 145.0 88 158.0 89 180.5 90 200.5 91 257.0 92 303.0 93 320.5 94 415.5 95 583.0 96 673.0 97 828.0 98 1525.0 99 25861.5 100 49656.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007628812499046398 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0022886437497139193 20-21 0.003814406249523199 22-23 0.0015257624998092796 24-25 0.014494743748188156 26-27 0.012968981248378877 28-29 0.022123556247234555 30-31 0.028989487496376313 32-33 0.028989487496376313 34-35 0.03204101249599487 36-37 0.03661829999542271 38-39 0.038144062495231994 40-41 0.026700843746662397 42-43 0.015257624998092797 44-45 0.004577287499427839 46-47 0.0030515249996185593 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0022886437497139193 56-57 0.003814406249523199 58-59 0.0 60-61 0.0 62-63 0.0022886437497139193 64-65 0.0 66-67 0.0 68-69 0.0015257624998092796 70-71 0.0061030499992371185 72-73 0.020597793747425274 74-75 0.034329656245708794 76 0.027463724996567032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 65541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.142292610732213 #Duplication Level Percentage of deduplicated Percentage of total 1 87.84119106699751 12.422758273447156 2 5.200129463804078 1.4708350498161458 3 2.427446326464559 1.0298896873712637 4 1.5643542992771604 0.8849422498893823 5 0.8738806775272414 0.6179338124227582 6 0.49627791563275436 0.4211104499473612 7 0.34523681087495955 0.34177079995727866 8 0.19419570611716475 0.21970979997253626 9 0.0647319020390549 0.0823911749897011 >10 0.8523033768475564 2.2596542622175435 >50 0.0647319020390549 0.6240368624219954 >100 0.05394325169921243 1.2892693123388412 >500 0.0 0.0 >1k 0.010788650339842486 3.692345249538457 >5k 0.0 0.0 >10k+ 0.010788650339842486 74.64335301566958 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 48922 74.64335301566958 No Hit CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG 2420 3.692345249538457 No Hit TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT 276 0.4211104499473612 No Hit ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC 172 0.26243114996719613 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 154 0.23496742497062909 No Hit CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG 140 0.21360674997329918 No Hit GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 103 0.1571535374803558 No Hit TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG 91 0.13884438748264444 No Hit GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 74 0.11290642498588671 No Hit TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 68 0.10375184998703102 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 66 0.10070032498741247 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0030515249996185593 0.0 2 0.0 0.0 0.0 0.0030515249996185593 0.0 3 0.0 0.0 0.0 0.0030515249996185593 0.0 4 0.0 0.0 0.0 0.0030515249996185593 0.0 5 0.0 0.0 0.0 0.0061030499992371185 0.0 6 0.0 0.0 0.0 0.0061030499992371185 0.0 7 0.0 0.0 0.0 0.0061030499992371185 0.0 8 0.0 0.0 0.0 0.007628812499046398 0.0 9 0.0 0.0 0.0 0.010680337498664958 0.0 10 0.0 0.0 0.0 0.010680337498664958 0.0 11 0.0 0.0 0.0 0.010680337498664958 0.0 12 0.0 0.0 0.0 0.010680337498664958 0.0 13 0.0 0.0 0.0 0.010680337498664958 0.0 14 0.0 0.0 0.0 0.010680337498664958 0.0 15 0.0 0.0 0.0 0.010680337498664958 0.0 16 0.0 0.0 0.0 0.010680337498664958 0.0 17 0.0 0.0 0.0 0.010680337498664958 0.0 18 0.0 0.0 0.0 0.019834912497520635 0.0 19 0.0 0.0 0.0 0.025937962496757755 0.0 20 0.0 0.0 0.0 0.030515249996185594 0.0 21 0.0 0.0 0.0 0.033566774995804155 0.0 22 0.0 0.0 0.0 0.04729863749408767 0.0 23 0.0 0.0 0.0 0.05340168749332479 0.0 24 0.0 0.0 0.0 0.05340168749332479 0.0 25 0.0 0.0 0.0 0.05340168749332479 0.0 26 0.0 0.0 0.0 0.05340168749332479 0.0 27 0.0 0.0 0.0 0.05645321249294335 0.0 28 0.0 0.0 0.0 0.07018507499122686 0.0 29 0.0 0.0 0.0 0.07323659999084542 0.0 30 0.0 0.0 0.0 0.08086541248989182 0.0 31 0.0 0.0 0.0 0.09154574998855679 0.0 32 0.0 0.0 0.0 0.10832913748645885 0.0 33 0.0 0.0 0.0 0.12511252498436093 0.0 34 0.0 0.0 0.0 0.14647319998169084 0.0 35 0.0 0.0 0.0 0.17546268747806718 0.0 36 0.0 0.0 0.0 0.20902946247387133 0.0 37 0.0 0.0 0.0 0.28379182496452604 0.0 38 0.0 0.0 0.0 0.3768633374528921 0.0 39 0.0 0.0 0.0 0.4775636624403045 0.0 40 0.0 0.0 0.0 0.6255626249218047 0.0 41 0.0 0.0 0.0 0.7079537999115058 0.0 42 0.0 0.0 0.0 0.8284890373964389 0.0 43 0.0 0.0 0.0 0.9185090248851864 0.0 44 0.0 0.0 0.0 1.0497245998687845 0.0 45 0.0 0.0 0.0 1.2068781373491402 0.0 46 0.0 0.0 0.0 1.2907950748386505 0.0 47 0.0 0.0 0.0 1.368608962328924 0.0 48 0.0 0.0 0.0 1.4540516623182436 0.0 49 0.0 0.0 0.0 1.5593292748050838 0.0 50 0.0 0.0 0.0 1.6401946872949758 0.0 51 0.0 0.0 0.0 1.7225858622846768 0.0 52 0.0 0.0 0.0 1.8095543247738057 0.0 53 0.0 0.0 0.0 1.908728887261409 0.0 54 0.0 0.0 0.0 1.9926458247509193 0.0 55 0.0 0.0 0.0 2.0658824247417646 0.0 56 0.0 0.0 0.0 2.1696342747287956 0.0 57 0.0 0.0 0.0 2.242870874719641 0.0 58 0.0 0.0 0.0 2.3115301872110585 0.0 59 0.0 0.0 0.0 2.3817152622022855 0.0 60 0.0 0.0 0.0 2.448848812193894 0.0 61 0.0 0.0 0.0 2.508353549686456 0.0 62 0.0 0.0 0.0 2.564806762179399 0.0 63 0.0 0.0 0.0 2.6868677621641415 0.0 64 0.0 0.0 0.0 2.7189087746601364 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTGTG 15 0.0022155235 70.0 8 TACTGGT 15 0.0022155235 70.0 31 AGTTGCT 15 0.0022155235 70.0 28 CGTGTGG 20 7.873018E-5 70.0 9 TGTGGCG 15 0.0022155235 70.0 11 ACTGGTG 15 0.0022155235 70.0 32 CCGGCGG 40 1.3278623E-10 70.0 21 TGCCGGC 40 1.0464646E-8 61.25 19 GCCGGCG 55 3.45608E-11 57.27273 20 TCTGGCG 45 2.651359E-8 54.444447 11 TAGTGTA 20 0.0069229505 52.5 34 GGCGTAG 20 0.0069229505 52.5 1 TATAAAC 20 0.0069229505 52.5 3 CTGGCGG 20 0.0069229505 52.5 12 CTGCCGG 40 7.375547E-7 52.5 18 TGGCGCG 20 0.0069229505 52.5 13 GTGTGGC 20 0.0069229505 52.5 10 CCGACGG 20 0.0069229505 52.5 21 AAAAAGG 225 0.0 51.333336 70 ATCATTA 350 0.0 48.0 59 >>END_MODULE