FastQCFastQC Report
Wed 25 May 2016
SRR1781021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65541
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT1723726.299568209212552No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT851912.997970735875253No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT37235.6804137867899485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36515.57055888680368No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG26163.991394699501076No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT14792.256602737217925No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT10521.605102149799362No Hit
CAAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT8371.2770632123403671No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4350.6637066874170366No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT4230.6453975374193254No Hit
CACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3930.599624662425047No Hit
ACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3290.501975862437253No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3050.4653575624418303No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2480.37838909995270137RNA PCR Primer, Index 15 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2440.37228604995346426No Hit
AAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2310.3524511374559436RNA PCR Primer, Index 15 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT2020.3082040249614745No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT1810.27616301246547964No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1730.26395691246700537No Hit
CAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1640.25022504996872186No Hit
TCTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1630.24869928746891257No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1520.2319158999710105No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1520.2319158999710105No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT1500.22886437497139195No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT1450.22123556247234558No Hit
ACTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1240.18919454997635068No Hit
CACTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1200.18309149997711355No Hit
CATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1140.17393692497825788No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC1140.17393692497825788No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT1120.17088539997863933No Hit
CATAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1080.1647823499794022No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT1050.16020506247997437No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT1040.15867929998016508No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1010.15410201248073724No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT1000.15257624998092797No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT970.14799896248150013No Hit
CATAAACACATCACCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT940.14342167498207228No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG910.13884438748264444No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT900.13731862498283517No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC850.12968981248378877No Hit
CATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC850.12968981248378877No Hit
CACATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC830.1266382874841702No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT820.12511252498436093No Hit
CATATACACATCACCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT810.12358676248455165No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT810.12358676248455165No Hit
AAACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT710.10832913748645885RNA PCR Primer, Index 15 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTAGA252.8111754E-670.0000155
ATCTACG252.8111754E-670.0000110
AAATATC252.8111754E-670.0000135
TCGAAAG252.8111754E-670.0000140
CAATCTC252.8111754E-670.0000135
TCTACGA252.8111754E-670.0000111
GCAGTCA252.8111754E-670.0000146
GCCGACA252.8111754E-670.0000146
GTTATCT252.8111754E-670.0000133
AGTTATC252.8111754E-670.0000132
GTTAAAA252.8111754E-670.0000133
CAGCCCA150.002215523570.016
CAGCTAG207.873018E-570.054
CTCGAAA207.873018E-570.039
CTATATA207.873018E-570.02
ACTCGAA150.002215523570.038
CAGTCAT301.0092663E-770.047
TAAACCC207.873018E-570.03
TATATAC207.873018E-570.03
TGCCGTA207.873018E-570.045
Produced by FastQC (version 0.11.5)