##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781021_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 65541 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.427655971071545 32.0 32.0 32.0 22.0 32.0 2 22.478250255565218 27.0 13.0 32.0 8.0 32.0 3 27.12354098960956 32.0 27.0 32.0 13.0 32.0 4 27.487786271189027 32.0 27.0 32.0 13.0 32.0 5 22.93976289650753 27.0 13.0 32.0 8.0 32.0 6 30.671793228666026 37.0 27.0 37.0 13.0 37.0 7 34.4688363009414 37.0 37.0 37.0 27.0 37.0 8 31.904914481011886 37.0 32.0 37.0 13.0 37.0 9 35.05428662974322 37.0 37.0 37.0 32.0 37.0 10-11 33.17991028516501 37.0 34.5 37.0 22.0 37.0 12-13 34.30739537083657 37.0 37.0 37.0 24.5 37.0 14-15 34.54733678155658 37.0 37.0 37.0 27.0 37.0 16-17 31.973199981690847 37.0 29.5 37.0 17.5 37.0 18-19 33.29367113715079 37.0 34.5 37.0 20.0 37.0 20-21 34.50546985856182 37.0 37.0 37.0 27.0 37.0 22-23 33.59518469355061 37.0 34.5 37.0 24.5 37.0 24-25 33.96978990250378 37.0 37.0 37.0 24.5 37.0 26-27 34.40369387101204 37.0 37.0 37.0 27.0 37.0 28-29 34.64968493004379 37.0 37.0 37.0 29.5 37.0 30-31 33.81896827939763 37.0 34.5 37.0 27.0 37.0 32-33 34.24917990265635 37.0 37.0 37.0 27.0 37.0 34-35 33.06969683099129 37.0 34.5 37.0 13.0 37.0 36-37 32.607291618986594 37.0 32.0 37.0 17.5 37.0 38-39 31.68889702628889 37.0 29.5 37.0 13.0 37.0 40-41 33.64501609679437 37.0 34.5 37.0 24.5 37.0 42-43 33.24097130040738 37.0 37.0 37.0 17.5 37.0 44-45 33.675729695915535 37.0 34.5 37.0 24.5 37.0 46-47 32.688012084039 37.0 32.0 37.0 17.5 37.0 48-49 31.201919409224757 37.0 27.0 37.0 10.5 37.0 50-51 32.83018263377123 37.0 34.5 37.0 17.5 37.0 52-53 32.397636593887796 37.0 32.0 37.0 13.0 37.0 54-55 32.963381700004575 37.0 32.0 37.0 22.0 37.0 56-57 31.811865854961017 37.0 29.5 37.0 13.0 37.0 58-59 32.208823484536396 37.0 32.0 37.0 13.0 37.0 60-61 32.29474679971316 37.0 29.5 37.0 17.5 37.0 62-63 34.039524877557554 37.0 34.5 37.0 27.0 37.0 64-65 34.323576082147056 37.0 37.0 37.0 27.0 37.0 66-67 34.08173509711479 37.0 37.0 37.0 27.0 37.0 68-69 33.07914892967761 37.0 32.0 37.0 17.5 37.0 70-71 29.400299049449963 34.5 20.0 37.0 13.0 37.0 72-73 30.895203002700597 37.0 29.5 37.0 13.0 37.0 74-75 32.42338383607208 37.0 32.0 37.0 13.0 37.0 76 33.342121725332234 37.0 37.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.03673488999288921 11101 2 0.7718521617989325 11101 3 0.004822610868647104 11101 4 -1.0784760174647694 11101 5 -0.2691103873054672 11101 6 -1.9380384977178196 11101 7 -2.339213235900367 11101 8 -1.414451322586455 11101 9 -1.076383404128208 11101 10-11 -1.054410944307719 11101 12-13 0.21936328222707857 11101 14-15 0.14657907755788102 11101 16-17 -0.6357000342717534 11101 18-19 0.21265756784128342 11101 20-21 -0.2884868382140766 11101 22-23 -1.2521171061076686 11101 24-25 -0.6210226110822035 11101 26-27 0.13297426565812032 11101 28-29 -0.32323144451754615 11101 30-31 0.3445226200690712 11101 32-33 -1.3179438462829793 11101 34-35 -0.3747791876707467 11101 36-37 -1.486613074767007 11101 38-39 0.14906459980704767 11101 40-41 -0.24393300014195773 11101 42-43 -0.6717828647763966 11101 44-45 0.1873397589738346 11101 46-47 -1.0027039691518524 11101 48-49 -0.6916217861821146 11101 50-51 -2.320502464984969 11101 52-53 -0.7616028281507425 11101 54-55 -0.8750132433608542 11101 56-57 -1.4042467528855624 11101 58-59 -1.2916453560415668 11101 60-61 -1.137657446111774 11101 62-63 -1.3180300931134568 11101 64-65 -1.3665979506145192 11101 66-67 -1.1646704962947538 11101 68-69 -2.20250637224607 11101 70-71 -2.086136496634669 11101 72-73 -0.5350389585556918 11101 74-75 -0.5935175078440729 11101 76 -0.5210828992838827 11102 1 0.11499575955810926 11102 2 1.9607410506878225 11102 3 0.568590726810676 11102 4 -0.7388624908947214 11102 5 0.23813598950612658 11102 6 -1.0187148262202363 11102 7 -0.08027603783273207 11102 8 -1.6835334481903175 11102 9 -0.2932916166885917 11102 10-11 -0.6215607027618262 11102 12-13 -1.1934386501400809 11102 14-15 -0.18409725094453222 11102 16-17 -0.7308691163973577 11102 18-19 -0.19144871235194927 11102 20-21 0.7827692004332647 11102 22-23 0.2174481112836233 11102 24-25 -1.4548390362029764 11102 26-27 3.6557000594683586E-4 11102 28-29 0.10309705789791224 11102 30-31 -0.6714194089164351 11102 32-33 -1.0667361168143756 11102 34-35 -0.9989337770427298 11102 36-37 -0.9571444757331946 11102 38-39 -0.0965875741059925 11102 40-41 -1.0347542561806122 11102 42-43 -1.2058408357908874 11102 44-45 -0.02739454054307089 11102 46-47 -2.0070517952388123 11102 48-49 -0.6493512547811484 11102 50-51 -0.5154300012168562 11102 52-53 -0.38551587162900347 11102 54-55 -0.7786364317666497 11102 56-57 -1.1003820185860462 11102 58-59 -0.4503410082154815 11102 60-61 -0.8562564799281986 11102 62-63 -0.5279334747559687 11102 64-65 0.36359528610045544 11102 66-67 0.19644061481636044 11102 68-69 -1.0911537152412407 11102 70-71 -1.0948321488085817 11102 72-73 -1.6637829199083498 11102 74-75 -1.0809571213706448 11102 76 -1.908039421023009 11103 1 -0.26326511000710795 11103 2 -0.7992589493121791 11103 3 1.3581559442019824 11103 4 -1.6562537952425451 11103 5 0.7242229460278651 11103 6 0.0508503911710676 11103 7 -0.5481021247892528 11103 8 0.027770899635768842 11103 9 -1.3941611819059858 11103 10-11 -0.23329983319661096 11103 12-13 -0.3595256066618191 11103 14-15 0.5254679664467758 11103 16-17 -0.08347781204953009 11103 18-19 -0.2462313210476097 11103 20-21 -1.4507090604362958 11103 22-23 0.5478828938923286 11103 24-25 -0.30657816663775606 11103 26-27 -0.4770257343418791 11103 28-29 0.4578796665935698 11103 30-31 -0.7788107132642637 11103 32-33 -0.7623882907274222 11103 34-35 0.5441097012181473 11103 36-37 -0.6510575192114487 11103 38-39 -0.9309354001929506 11103 40-41 0.028289222080260856 11103 42-43 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11104 22-23 0.06216860817804104 11104 24-25 0.45199326193367284 11104 26-27 -0.312263829579976 11104 28-29 0.19264157135547322 11104 30-31 0.12309404864049611 11104 32-33 1.0352307568916288 11104 34-35 -0.8477950606866145 11104 36-37 -0.1072479954019272 11104 38-39 -1.7699830192405699 11104 40-41 -1.538377444586402 11104 42-43 0.49964570665218133 11104 44-45 -0.8094856378515658 11104 46-47 0.9163436498957651 11104 48-49 -0.1519392464995768 11104 50-51 -0.8109786554611595 11104 52-53 0.8479209813730648 11104 54-55 0.6654629471153442 11104 56-57 -0.9026594512982591 11104 58-59 -0.6630739274701369 11104 60-61 -0.46702252547685674 11104 62-63 0.2645095894262255 11104 64-65 0.7341957001791286 11104 66-67 -1.4464165280407784 11104 68-69 -0.7112365309762296 11104 70-71 -1.9742317347299085 11104 72-73 0.6736912001744635 11104 74-75 -0.384787349113914 11104 76 1.0360599578589742 11105 1 -1.232830327398414 11105 2 0.9607410506878225 11105 3 -0.8444527514501949 11105 4 -0.6301668387208075 11105 5 0.06422294602786494 11105 6 2.111719956388459 11105 7 1.811028309993354 11105 8 -0.9661421438424931 11105 9 -0.2932916166885917 11105 10-11 -0.1867780940661703 11105 12-13 -0.7043082153574716 11105 14-15 -0.48844507703148565 11105 16-17 0.0734787096896028 11105 18-19 1.79768172243066 11105 20-21 1.4023344178245765 11105 22-23 0.03266550258798162 11105 24-25 -0.32440425359428104 11105 26-27 -0.2931126908636159 11105 28-29 0.2878796665935681 11105 30-31 0.18727624325747172 11105 32-33 0.19413344840301505 11105 34-35 0.5880227446964099 11105 36-37 0.49937726339724264 11105 38-39 1.9577602519809645 11105 40-41 1.030463135123746 11105 42-43 1.098506990296066 11105 44-45 -0.027394540543077994 11105 46-47 -0.8113996213257693 11105 48-49 0.6767357017405864 11105 50-51 0.24543956400053446 11105 52-53 -0.0268202194550895 11105 54-55 -1.8221146926362124 11105 56-57 -0.5895124533686555 11105 58-59 -0.9286018777807001 11105 60-61 2.3502652592022315 11105 62-63 0.7981534817657661 11105 64-65 1.1244648513178532 11105 66-67 -0.7709506895314675 11105 68-69 -0.04767545437167797 11105 70-71 -1.0187451922868398 11105 72-73 1.9123040366133885 11105 74-75 1.5059994003684878 11105 76 1.4615257963682922 11106 1 0.35102060427860593 11106 2 1.1988362887830597 11106 3 1.3429178489638858 11106 4 -0.33720617619492543 11106 5 -1.935777053972135 11106 6 -1.1015305612098842 11106 7 0.35761216092502934 11106 8 1.326818518683389 11106 9 0.1839340561892584 11106 10-11 -0.512347452244228 11106 12-13 0.039998202861994514 11106 14-15 0.5426108235896336 11106 16-17 -0.0491920977638145 11106 18-19 -0.8524217972380796 11106 20-21 -0.046423346150582745 11106 22-23 0.44312098913042064 11106 24-25 -0.08372102378061896 11106 26-27 0.6044028370866883 11106 28-29 0.4783558570697579 11106 30-31 0.3849988105452553 11106 32-33 0.2852307568916288 11106 34-35 -0.4549379178294757 11106 36-37 0.6308472426933065 11106 38-39 -0.9723639716215189 11106 40-41 -0.585996492205453 11106 42-43 -0.714640007633534 11106 44-45 1.1548000764341495 11106 46-47 0.1663436498957651 11106 48-49 -1.1281297226900513 11106 50-51 0.2128308683483624 11106 52-53 -0.009221875769796384 11106 54-55 -0.060727529075137454 11106 56-57 -0.22408802272683204 11106 58-59 0.015497501101293665 11106 60-61 -1.0384510969054261 11106 62-63 -0.9973951724785337 11106 64-65 -0.9800900141065796 11106 66-67 -0.42260700423125996 11106 68-69 0.40781108807139077 11106 70-71 -1.295660306158478 11106 72-73 -1.2905945141112518 11106 74-75 -1.2181206824472461 11106 76 0.8932028150018354 11107 1 -0.7991625459045437 11107 2 0.20433079427756695 11107 3 -0.7797927737467383 11107 4 1.671951332962582 11107 5 -3.0383411565362373 11107 6 -1.3927393524186762 11107 7 -2.5306662273533576 11107 8 0.31033500219987076 11107 9 0.0484029206581198 11107 10-11 0.27977708988031225 11107 12-13 0.5509872138510019 11107 14-15 -0.20555767457886986 11107 16-17 0.883958085386368 11107 18-19 -0.5300774748937584 11107 20-21 0.96364991392268 11107 22-23 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-1.935777053972135 11108 6 1.0889456292663056 11108 7 0.45285025616312424 11108 8 0.08872328058814816 11108 9 1.2553626276178278 11108 10-11 0.6543192144224435 11108 12-13 1.266188679052469 11108 14-15 0.25689653787534183 11108 16-17 1.1412840927123753 11108 18-19 1.5523401075238255 11108 20-21 0.7988147490875122 11108 22-23 -0.03306948706005386 11108 24-25 1.4996123095527238 11108 26-27 0.5567837894676444 11108 28-29 0.7164510951649987 11108 30-31 0.2421416676881165 11108 32-33 0.9757069473678186 11108 34-35 2.4022049393133855 11108 36-37 0.8213234331694963 11108 38-39 1.2776360283784811 11108 40-41 1.0449558887469337 11108 42-43 1.4401218971283711 11108 44-45 0.226228647862726 11108 46-47 0.9282484118005243 11108 48-49 0.7766321820718467 11108 50-51 1.1175927731102675 11108 52-53 0.20506383851592602 11108 54-55 -0.13215610050370685 11108 56-57 -0.43837373701254734 11108 58-59 0.9440689296727243 11108 60-61 1.4139298554755229 11108 62-63 1.3240333989500357 11108 64-65 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44-45 -0.3699251982911278 11109 46-47 0.8998601334122469 11109 48-49 0.1502585556982261 11109 50-51 0.7000103555278514 11109 52-53 -0.11544898199689868 11109 54-55 -0.13398760233521045 11109 56-57 0.6248130761742665 11109 58-59 0.21604695165073906 11109 60-61 -0.12911043756476914 11109 62-63 -0.4607651358485043 11109 64-65 -0.457196241212813 11109 66-67 0.5618252302009736 11109 68-69 0.4838184140787192 11109 70-71 0.847196836698668 11109 72-73 -0.7777740012907373 11109 74-75 1.4970807827542174 11109 76 2.7814812032802223 11110 1 -2.3876553539095475 11110 2 -0.4173077297999832 11110 3 -0.5008684460419204 11110 4 1.5681364486598923 11110 5 -1.3748014442160361 11110 6 1.228899171658874 11110 7 -0.03785822235023062 11110 8 0.6467952898796696 11110 9 1.2141315010208444 11110 10-11 0.10060260582778113 11110 12-13 0.0597426860211101 11110 14-15 1.1183947957150693 11110 16-17 -1.5622582998544061 11110 18-19 0.5871833130987412 11110 20-21 0.9405104517588256 11110 22-23 -0.688702471961335 11110 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28-29 -0.4621203334064319 21612 30-31 -0.4669357132642631 21612 32-33 -0.7780132907274222 21612 34-35 -3.2733902987818553 21612 36-37 -0.6623075192114527 21612 38-39 1.3803145998070505 21612 40-41 -1.2467107779197377 21612 42-43 -0.41032453144305947 21612 44-45 0.1533119811960546 21612 46-47 0.2660460308481447 21612 48-49 0.3309476582623283 21612 50-51 1.1034558683483624 21612 52-53 2.3977721718492546 21612 54-55 1.370820089972483 21612 56-57 -0.8795939751077846 21612 58-59 0.9864796439584325 21612 60-61 -0.9119630016673312 21612 62-63 0.9482893513309847 21612 64-65 0.4097909382743694 21612 66-67 0.41519061481636044 21612 68-69 0.99784084997615 21612 70-71 -1.4392614966346677 21612 72-73 -1.394761180777916 21612 74-75 -0.9532397300662936 21612 76 -0.7273328992838799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 3.0 18 5.0 19 25.0 20 98.0 21 243.0 22 422.0 23 713.0 24 1042.0 25 1399.0 26 1860.0 27 2271.0 28 2719.0 29 3131.0 30 3599.0 31 4294.0 32 5217.0 33 7211.0 34 11258.0 35 16135.0 36 3894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 8.25780463242699 6.0392444078244685 7.836674906161312 77.86627605358724 2 7.283990174089501 57.007064280374124 23.722555347034678 11.9863901985017 3 7.737141636532857 18.336613722707924 68.86223890389222 5.064005736866999 4 6.826261424146718 74.9744434781282 13.12918631085885 5.0701087868662364 5 6.928487511633939 33.54694008330663 56.62714941792161 2.897422987137822 6 8.228688261950898 77.07014144249989 9.940646027556111 4.7605242679931035 7 7.8806835520292955 15.262435154104365 5.292950869697894 71.56393042416845 8 7.54031827405746 76.35831006545521 3.34752292458156 12.753848735905768 9 7.628812499046399 12.692818235913398 2.001800399749775 77.67656886529043 10-11 7.850048061518744 44.37451366320319 37.39033582032621 10.385102454951863 12-13 8.537404067682825 6.054225599243222 42.59394882592575 42.8144215071482 14-15 9.552036130055995 4.1737233182282845 8.052974473993379 78.22126607772233 16-17 47.66710913779161 37.68175645778978 1.8087914435239012 12.842342960894706 18-19 48.90333310446372 10.233523295112109 1.85994705482953 39.00319654559464 20-21 10.710852748661145 4.499473611937566 1.8377809310202775 82.95189270838101 22-23 9.561953586304755 42.90596725713676 1.7431836560321021 45.78889550052639 24-25 47.76857234402893 40.67987976991502 1.885079568514365 9.666468317541693 26-27 48.9815535313773 45.0664469568667 2.8272379121465954 3.1247615996094047 28-29 9.902349710100703 46.056606652426005 2.9600244125724746 41.08101922490082 30-31 8.906978340975153 45.67010230627799 41.254377198135444 4.168542154611412 32-33 42.772463038403444 48.84576066889428 6.045071024244366 2.3367052684579117 34-35 14.288765810713905 9.10575059886178 74.42516897819685 2.180314612227461 36-37 7.842419249019697 76.44756717169405 12.424284035946965 3.2857295433392837 38-39 8.526723730184159 13.025434460871821 41.05674310736791 37.39109870157611 40-41 8.795526327835335 4.286760554784174 43.43596942278643 43.48174369459406 42-43 47.16616950342165 4.225760430891766 39.84833342233954 8.759736643347042 44-45 10.67576021116553 41.91269586976091 43.29656245708793 4.114981461985627 46-47 47.04186025221431 5.955950228488164 9.656008971688829 37.34618054760869 48-49 44.46360182486764 3.9999084514563847 4.623964357100353 46.91252536657562 50-51 13.649471323293817 4.2461970369692255 42.03017958224623 40.07415205749073 52-53 8.182664286477168 5.057139805617857 77.079995727865 9.680200180039975 54-55 8.433652217695792 4.4269998931966255 45.69277246303841 41.446575426069174 56-57 46.50066370668742 3.6015623807998045 10.650585129918678 39.2471887825941 58-59 10.469258996483036 7.410798068340467 73.58158057354724 8.538362361629247 60-61 45.872049556765994 41.235257319845594 10.33551517370806 2.557177949680353 62-63 9.272058711340993 86.09420057673822 2.2039639309745045 2.429776780946278 64-65 7.300010680337499 88.40039059519995 1.9102546497612183 2.389344074701332 66-67 7.395370836575578 88.27375230771578 1.8888939747638882 2.441982880944752 68-69 9.104987717611875 86.43978578294502 1.8560900810179888 2.599136418425108 70-71 42.611495094673565 52.52437405593445 2.1040264872369967 2.760104362154987 72-73 89.82895680434551 5.142739437586781 2.3009200628633333 2.727383695204382 74-75 92.72058711340992 2.6716101371660486 2.0429959872446255 2.564806762179399 76 93.03641995087045 2.532765749683404 2.0552020872431 2.3756122122030483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 1.0 16 2.5 17 3.0 18 5.0 19 8.0 20 9.5 21 10.0 22 17.0 23 21.0 24 14.5 25 11.0 26 18.5 27 35.0 28 53.0 29 62.0 30 81.0 31 117.0 32 159.5 33 185.0 34 210.0 35 284.0 36 371.5 37 410.0 38 530.5 39 829.0 40 1311.5 41 2542.5 42 3469.0 43 7168.0 44 23407.0 45 19121.5 46 2296.0 47 1640.5 48 747.5 49 398.5 50 287.0 51 258.5 52 230.0 53 231.0 54 232.0 55 221.5 56 215.5 57 204.5 58 189.0 59 192.5 60 197.0 61 169.0 62 140.0 63 141.5 64 123.5 65 92.5 66 92.0 67 103.0 68 78.5 69 53.0 70 52.5 71 53.0 72 45.5 73 26.0 74 14.0 75 14.0 76 10.5 77 9.5 78 9.5 79 7.0 80 6.5 81 4.0 82 2.5 83 3.0 84 2.5 85 1.0 86 0.5 87 1.0 88 2.0 89 2.5 90 1.5 91 2.0 92 3.0 93 4.0 94 8.0 95 10.0 96 9.0 97 19.5 98 56.0 99 1843.0 100 3604.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004577287499427839 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0030515249996185593 7 0.0015257624998092796 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0022886437497139193 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0015257624998092796 30-31 0.003814406249523199 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0030515249996185593 42-43 0.003814406249523199 44-45 0.0 46-47 0.0022886437497139193 48-49 0.0030515249996185593 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0022886437497139193 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0030515249996185593 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 65541.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.311575960086053 #Duplication Level Percentage of deduplicated Percentage of total 1 84.08785652208263 16.238690285470163 2 7.276605830765584 2.8104545246486934 3 3.1445050169866477 1.8217604247722798 4 1.5406494429959707 1.1900947498512382 5 0.892786600300229 0.862055812392243 6 0.56095441257802 0.6499748249187531 7 0.34763372047088564 0.46993484994125817 8 0.22912222485581102 0.3539768999557529 9 0.22912222485581102 0.398224012450222 >10 1.2167180216481 4.925161349384355 >50 0.20541992573279605 2.831815199646023 >100 0.20541992573279605 7.769182649028853 >500 0.00790076637433831 1.2770632123403671 >1k 0.039503831871691555 19.10407226011199 >5k 0.00790076637433831 12.997970735875253 >10k+ 0.00790076637433831 26.299568209212552 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 17237 26.299568209212552 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 8519 12.997970735875253 No Hit CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 3723 5.6804137867899485 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 3651 5.57055888680368 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 2616 3.991394699501076 No Hit CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 1479 2.256602737217925 No Hit CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 1052 1.605102149799362 No Hit CAAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 837 1.2770632123403671 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 435 0.6637066874170366 No Hit CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 423 0.6453975374193254 No Hit CACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 393 0.599624662425047 No Hit ACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 329 0.501975862437253 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 305 0.4653575624418303 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 248 0.37838909995270137 RNA PCR Primer, Index 15 (95% over 21bp) CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 244 0.37228604995346426 No Hit AAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 231 0.3524511374559436 RNA PCR Primer, Index 15 (95% over 21bp) CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT 202 0.3082040249614745 No Hit CATATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT 181 0.27616301246547964 No Hit TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 173 0.26395691246700537 No Hit CAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 164 0.25022504996872186 No Hit TCTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 163 0.24869928746891257 No Hit CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 152 0.2319158999710105 No Hit CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 152 0.2319158999710105 No Hit CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT 150 0.22886437497139195 No Hit CATATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 145 0.22123556247234558 No Hit ACTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 124 0.18919454997635068 No Hit CACTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 120 0.18309149997711355 No Hit CATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 114 0.17393692497825788 No Hit CATATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC 114 0.17393692497825788 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT 112 0.17088539997863933 No Hit CATAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 108 0.1647823499794022 No Hit TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT 105 0.16020506247997437 No Hit CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT 104 0.15867929998016508 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 101 0.15410201248073724 No Hit CATATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT 100 0.15257624998092797 No Hit CATAAACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT 97 0.14799896248150013 No Hit CATAAACACATCACCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 94 0.14342167498207228 No Hit CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 91 0.13884438748264444 No Hit CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT 90 0.13731862498283517 No Hit CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 85 0.12968981248378877 No Hit CATTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC 85 0.12968981248378877 No Hit CACATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 83 0.1266382874841702 No Hit CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT 82 0.12511252498436093 No Hit CATATACACATCACCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 81 0.12358676248455165 No Hit CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT 81 0.12358676248455165 No Hit AAACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 71 0.10832913748645885 RNA PCR Primer, Index 15 (95% over 21bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0061030499992371185 0.0 6 0.0 0.0 0.0 0.0061030499992371185 0.0 7 0.0 0.0 0.0 0.0061030499992371185 0.0 8 0.0 0.0 0.0 0.007628812499046398 0.0 9 0.0 0.0 0.0 0.012206099998474237 0.0 10 0.0 0.0 0.0 0.012206099998474237 0.0 11 0.0 0.0 0.0 0.012206099998474237 0.0 12 0.0 0.0 0.0 0.012206099998474237 0.0 13 0.0 0.0 0.0 0.012206099998474237 0.0 14 0.0 0.0 0.0 0.012206099998474237 0.0 15 0.0 0.0 0.0 0.013731862498283518 0.0 16 0.0 0.0 0.0 0.013731862498283518 0.0 17 0.0 0.0 0.0 0.013731862498283518 0.0 18 0.0 0.0 0.0 0.03204101249599487 0.0 19 0.0 0.0 0.0 0.04272134999465983 0.0 20 0.0 0.0 0.0 0.04882439999389695 0.0 21 0.0 0.0 0.0 0.05187592499351551 0.0 22 0.0 0.0 0.0 0.05492744999313407 0.0 23 0.0 0.0 0.0 0.06255626249218046 0.0 24 0.0 0.0 0.0 0.06255626249218046 0.0 25 0.0 0.0 0.0 0.06255626249218046 0.0 26 0.0 0.0 0.0 0.06255626249218046 0.0 27 0.0 0.0 0.0 0.06560778749179903 0.0 28 0.0 0.0 0.0 0.07933964999008254 0.0 29 0.0 0.0 0.0 0.0823911749897011 0.0 30 0.0 0.0 0.0 0.0900199874887475 0.0 31 0.0 0.0 0.0 0.09154574998855679 0.0 32 0.0 0.0 0.0 0.11443218748569597 0.0 33 0.0 0.0 0.0 0.12968981248378877 0.0 34 0.0 0.0 0.0 0.15410201248073724 0.0 35 0.0 0.0 0.0 0.18614302497673213 0.0 36 0.0 0.0 0.0 0.2258128499717734 0.0 37 0.0 0.0 0.0 0.32193588745975804 0.0 38 0.0 0.0 0.0 0.4150073999481241 0.0 39 0.0 0.0 0.0 0.5141819624357272 0.0 40 0.0 0.0 0.0 0.6621809249172274 0.0 41 0.0 0.0 0.0 0.7506751499061656 0.0 42 0.0 0.0 0.0 0.8803649623899544 0.0 43 0.0 0.0 0.0 0.9749622373781297 0.0 44 0.0 0.0 0.0 1.1046520498619186 0.0 45 0.0 0.0 0.0 1.2618055873422742 0.0 46 0.0 0.0 0.0 1.3487740498314031 0.0 47 0.0 0.0 0.0 1.4326909873209137 0.0 48 0.0 0.0 0.0 1.5242367373094705 0.0 49 0.0 0.0 0.0 1.6325658747959293 0.0 50 0.0 0.0 0.0 1.7210600997848675 0.0 51 0.0 0.0 0.0 1.8019255122747593 0.0 52 0.0 0.0 0.0 1.8995743122625532 0.0 53 0.0 0.0 0.0 2.0033261622495844 0.0 54 0.0 0.0 0.0 2.0902946247387133 0.0 55 0.0 0.0 0.0 2.180314612227461 0.0 56 0.0 0.0 0.0 2.282540699714682 0.0 57 0.0 0.0 0.0 2.3557772997055277 0.0 58 0.0 0.0 0.0 2.416807799697899 0.0 59 0.0 0.0 0.0 2.4915701621885535 0.0 60 0.0 0.0 0.0 2.5556521871805433 0.0 61 0.0 0.0 0.0 2.6075281121740588 0.0 62 0.0 0.0 0.0 2.6624555621671933 0.0 63 0.0 0.0 0.0 2.7906196121511724 0.0 64 0.0 0.0 0.0 2.81655757464793 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTAGA 25 2.8111754E-6 70.00001 55 ATCTACG 25 2.8111754E-6 70.00001 10 AAATATC 25 2.8111754E-6 70.00001 35 TCGAAAG 25 2.8111754E-6 70.00001 40 CAATCTC 25 2.8111754E-6 70.00001 35 TCTACGA 25 2.8111754E-6 70.00001 11 GCAGTCA 25 2.8111754E-6 70.00001 46 GCCGACA 25 2.8111754E-6 70.00001 46 GTTATCT 25 2.8111754E-6 70.00001 33 AGTTATC 25 2.8111754E-6 70.00001 32 GTTAAAA 25 2.8111754E-6 70.00001 33 CAGCCCA 15 0.0022155235 70.0 16 CAGCTAG 20 7.873018E-5 70.0 54 CTCGAAA 20 7.873018E-5 70.0 39 CTATATA 20 7.873018E-5 70.0 2 ACTCGAA 15 0.0022155235 70.0 38 CAGTCAT 30 1.0092663E-7 70.0 47 TAAACCC 20 7.873018E-5 70.0 3 TATATAC 20 7.873018E-5 70.0 3 TGCCGTA 20 7.873018E-5 70.0 45 >>END_MODULE