FastQCFastQC Report
Wed 25 May 2016
SRR1781020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94125
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3119533.14209827357238No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG15171.611686586985392No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC1360.1444887118193891No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT1270.13492695883134131No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG1070.11367861885790173No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTAC150.00221500370.02817522
TGTGCGG207.875175E-570.0281753
ATAGTCC150.00221500370.02817556
TAGAGTG150.002219680569.990974
TCGATTC150.002219680569.9909747
TATTCCC150.002219680569.990974
CGTACGT150.002219680569.990978
GGTTCGA150.002219680569.9909744
CTCAATT200.00692128352.52113320
GGTAATG200.006935856352.49322539
CACGGAG200.006935856352.49322516
TAGGTCA200.006935856352.49322512
GCACGGA200.006935856352.49322515
CGATTCT200.006935856352.49322548
ATGTCGG200.006935856352.49322546
GTTCGAT200.006935856352.49322545
CGTAGGT200.006935856352.49322569
ACAATGT200.006935856352.49322546
GTTTTGG200.006935856352.4932254
GTATAGC200.006935856352.4932256
Produced by FastQC (version 0.11.5)