FastQCFastQC Report
Wed 25 May 2016
SRR1781020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94125
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1051511.171314741035857No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT54545.794422310756972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24042.554050464807437No Hit
CATAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT22212.3596281540504647No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG16181.7189907038512617No Hit
CAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT9551.0146082337317397No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT7190.7638778220451528No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT5270.5598937583001328No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3680.3909694555112882No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3330.3537848605577689No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2720.2889774236387782No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2470.26241699867197876Illumina PCR Primer Index 9 (95% over 21bp)
AAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2120.22523240371845948Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2080.22098273572377156No Hit
ACTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1820.19335989375830015No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1410.149800796812749No Hit
TCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1280.13598937583001328No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT1170.12430278884462151No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1160.12324037184594955No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG990.1051792828685259No Hit
CAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC990.1051792828685259No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAA207.8728066E-570.032439
AGCCGAC150.002219145469.99522445
GTTTACC150.002219145469.99522450
ATCAAGT150.002219145469.99522437
ATTCCGT150.002219145469.99522444
GGTTGCA150.002219145469.99522415
GCTTCTC207.893579E-569.9952268
CGAATGC950.066.3112641
CATATAC12700.065.034931
AGGGGGG26300.064.8054570
ATATACA12750.064.779892
GCCGACT650.064.6109746
CGTCTAC550.063.63201548
AAAGATC453.783498E-1062.2179733
CGAGCAC401.0535587E-861.24581515
TAAGCCG750.060.66251843
CATAAAC4100.059.7520181
TAAACAC5000.059.527563
CCGTCAT1000.059.49593447
GTCTACT650.059.22672349
Produced by FastQC (version 0.11.5)