FastQCFastQC Report
Wed 25 May 2016
SRR1781019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76507
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1669821.82545388003712No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG10921.427320375913315No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC1070.13985648372044387No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT1050.13724234383781878No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG830.10848680512894245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA150.002217855869.992811
ACAGGCA200.00691228552.5289431
CGCTTGT200.00691228552.5289435
GTAGCGA200.006930196752.49460649
GTTCAAA200.006930196752.49460639
CCGCGTC200.006930196752.49460617
CTTTACC200.006930196752.49460661
GAGGCTA200.006930196752.49460624
TCAGGGA305.847826E-446.66187741
TCGCCGT1800.042.77338452
CGCCGTA1800.042.77338453
GCCGTAT1800.042.77338454
ATCTCGG1900.042.3640742
AGATCTC1900.042.3640740
GATCTCG1900.042.3640741
GTCGCCG1850.041.61734851
TGGTCGC1850.041.61734849
TCTCGGT1950.041.27781343
CGTATCA1900.040.52215256
ATTAAAA2000.040.2458762