FastQCFastQC Report
Wed 25 May 2016
SRR1781019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76507
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT48986.402028572548916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17832.3305057053602938No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT15422.0155018495039667No Hit
CATAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT15161.9815180310298404No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG5490.7175813977805953No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT5480.7162743278392827No Hit
CAAAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT3900.5097572771118983No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2620.3424523246238906No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT1220.1594625328401323RNA PCR Primer, Index 30 (95% over 22bp)
CACTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1170.15292718313356948No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT1130.14769890336831926No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC890.11632922477681781No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT870.11371508489419269No Hit
ACTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG860.11240801495288012No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT840.10979387507025501No Hit
AAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT800.10456559530500477RNA PCR Primer, Index 30 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA650.070.0000146
ACTGCTT207.881479E-570.053
TGCTTAA150.002216952970.055
ACATTGA353.6525307E-970.027
CGTCTAC252.8157083E-670.048
TGACGGA353.6525307E-970.031
ATCCGAG301.0115582E-770.012
TTGACGG301.0115582E-770.030
CCGACTT252.8157083E-670.047
CGTAATC500.070.048
CGTAAGA207.881479E-570.041
CAAGAAT150.002216952970.01
CGACTTC150.002216952970.048
ATGCCGG353.6525307E-970.030
ATTGACG353.6525307E-970.029
CGTCATA207.881479E-570.048
TAAGACG150.002216952970.043
TGCCGAC353.6525307E-970.045
CGGATAT600.064.16666434
TGCCGTA1050.063.33333645
Produced by FastQC (version 0.11.5)