##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781019_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 76507 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.482687858627315 32.0 22.0 32.0 13.0 32.0 2 23.678408511639457 27.0 13.0 32.0 8.0 32.0 3 27.20859529193407 32.0 27.0 32.0 13.0 32.0 4 27.191172049616377 32.0 27.0 32.0 13.0 32.0 5 24.478936567895747 27.0 13.0 32.0 8.0 32.0 6 30.486321513064166 37.0 27.0 37.0 13.0 37.0 7 31.832564340517862 37.0 32.0 37.0 13.0 37.0 8 31.45414145110905 37.0 32.0 37.0 13.0 37.0 9 33.96605539362411 37.0 37.0 37.0 27.0 37.0 10-11 32.54966212242017 37.0 32.0 37.0 17.5 37.0 12-13 33.166671023566465 37.0 32.0 37.0 22.0 37.0 14-15 33.511181983347925 37.0 32.0 37.0 22.0 37.0 16-17 32.54030350164037 37.0 32.0 37.0 17.5 37.0 18-19 32.952429189485926 37.0 32.0 37.0 17.5 37.0 20-21 33.26955703399689 37.0 32.0 37.0 22.0 37.0 22-23 33.000960696406864 37.0 32.0 37.0 17.5 37.0 24-25 32.83424392539244 37.0 32.0 37.0 17.5 37.0 26-27 33.188956566065855 37.0 34.5 37.0 17.5 37.0 28-29 33.319572065301216 37.0 32.0 37.0 22.0 37.0 30-31 32.848530199851 37.0 32.0 37.0 13.0 37.0 32-33 32.574810148091025 37.0 32.0 37.0 13.0 37.0 34-35 32.16120747121178 37.0 32.0 37.0 13.0 37.0 36-37 32.316689976080625 37.0 32.0 37.0 13.0 37.0 38-39 31.908459356660174 37.0 32.0 37.0 13.0 37.0 40-41 31.255185799992155 37.0 27.0 37.0 13.0 37.0 42-43 31.250807115688758 37.0 29.5 37.0 13.0 37.0 44-45 32.03329107140523 37.0 32.0 37.0 13.0 37.0 46-47 31.00800580339054 37.0 27.0 37.0 13.0 37.0 48-49 30.728913694171773 37.0 29.5 37.0 10.5 37.0 50-51 30.667102356647106 37.0 27.0 37.0 13.0 37.0 52-53 30.55854366267139 37.0 27.0 37.0 13.0 37.0 54-55 30.185009214843085 37.0 27.0 37.0 13.0 37.0 56-57 30.153580718104223 37.0 24.5 37.0 13.0 37.0 58-59 30.475616610244813 37.0 24.5 37.0 13.0 37.0 60-61 30.73348843896637 37.0 27.0 37.0 13.0 37.0 62-63 31.95484726887736 37.0 32.0 37.0 13.0 37.0 64-65 32.1532539506189 37.0 32.0 37.0 13.0 37.0 66-67 32.03205589031069 37.0 32.0 37.0 13.0 37.0 68-69 31.575411400264027 37.0 27.0 37.0 13.0 37.0 70-71 29.683401518815273 37.0 24.5 37.0 13.0 37.0 72-73 30.129484883736126 37.0 24.5 37.0 13.0 37.0 74-75 31.0791692263453 37.0 27.0 37.0 13.0 37.0 76 30.999934646502933 37.0 27.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -1.0683758762997222 11101 2 -0.5619012601409352 11101 3 1.063446894045594 11101 4 1.618551117818864 11101 5 0.3807989124898157 11101 6 -1.0016363417664849 11101 7 -0.5346623802592951 11101 8 1.5279189432267977 11101 9 0.14933544986528346 11101 10-11 1.0485079435962419 11101 12-13 0.8522304915595527 11101 14-15 0.6750090618697087 11101 16-17 0.3749964464670157 11101 18-19 -0.6926428486300296 11101 20-21 0.07299779925516958 11101 22-23 0.8043966541536491 11101 24-25 -1.3452088359902135 11101 26-27 0.24093650728846683 11101 28-29 1.970892023421996 11101 30-31 0.5323901258534107 11101 32-33 1.6557571220533447 11101 34-35 0.2785934180094003 11101 36-37 0.5543193602137038 11101 38-39 0.9585602391691097 11101 40-41 -0.8514463473564042 11101 42-43 -0.6387069738435933 11101 44-45 0.5306333358444313 11101 46-47 -0.31704658825105625 11101 48-49 1.565695179846557 11101 50-51 -1.9019647617999738 11101 52-53 -1.6117020503100967 11101 54-55 0.4730895322285704 11101 56-57 -0.5673351389534282 11101 58-59 0.7092350807815251 11101 60-61 1.161205183321993 11101 62-63 -1.3126031647692749 11101 64-65 -0.4427411073396357 11101 66-67 -0.16862911144092863 11101 68-69 1.529844762799332 11101 70-71 -1.3837185031627648 11101 72-73 -0.2009893601932049 11101 74-75 -0.01391025968629478 11101 76 -0.7932658574142124 11102 1 0.5717756388517934 11102 2 0.7543866186469437 11102 3 0.4138256819243793 11102 4 1.6355965723643173 11102 5 -0.5945798753889697 11102 6 -0.3595908872210316 11102 7 0.22859519549828278 11102 8 -2.3262477234398666 11102 9 1.0716839347137679 11102 10-11 -1.3179693291310315 11102 12-13 0.12969261277167732 11102 14-15 -0.3420363926757446 11102 16-17 -0.6714050686844999 11102 18-19 -0.1803322425694276 11102 20-21 0.6601190113763806 11102 22-23 -0.2543154670584755 11102 24-25 0.5421017700703885 11102 26-27 0.8962395375914909 11102 28-29 0.6432405082704804 11102 30-31 0.14981436827764583 11102 32-33 0.6406056069018291 11102 34-35 0.31931311497909576 11102 36-37 -0.5602639731196248 11102 38-39 -0.25166703355816367 11102 40-41 -0.3315599837200409 11102 42-43 0.31583848070185994 11102 44-45 -0.09341969445859988 11102 46-47 -1.0850390124934854 11102 48-49 0.28444517984655704 11102 50-51 -0.40101779210300137 11102 52-53 -1.0709823533403977 11102 54-55 -0.19073622534718382 11102 56-57 1.2139148610465718 11102 58-59 0.19787144441789195 11102 60-61 -0.40981754395073366 11102 62-63 -2.5720728617389774 11102 64-65 -0.39823353158206487 11102 66-67 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0.24056395596663194 11103 48-49 -0.3380037997452803 11103 50-51 0.051904285819077245 11103 52-53 0.011809854451808377 11103 54-55 1.4307851104598655 11103 56-57 -1.1916973838513876 11103 58-59 -0.4693363477899055 11103 60-61 -1.79478971463719 11103 62-63 1.2686043182239253 11103 64-65 -0.11854893046889003 11103 66-67 -0.22581448559058614 11103 68-69 0.12019340225511499 11103 70-71 -1.7674344895573277 11103 72-73 -1.669739360193205 11103 74-75 -0.7783660419992202 11103 76 1.9813940065313638 11104 1 -0.7855744578600081 11104 2 2.07595334978814 11104 3 1.709280227378926 11104 4 0.8246681390954613 11104 5 0.18443366426286545 11104 6 0.39331046674415404 11104 7 -2.637942522103266 11104 8 -1.2358221915249743 11104 9 0.9396723292979061 11104 10-11 -0.268646311722911 11104 12-13 -0.7769805013482483 11104 14-15 -0.3954696615345412 11104 16-17 0.13807268760176328 11104 18-19 -2.038165898275423 11104 20-21 -0.3986334064185897 11104 22-23 -1.0710466082577028 11104 24-25 -0.4194552898909194 11104 26-27 -1.0790989537044453 11104 28-29 0.2745267751950493 11104 30-31 -0.6608722854941078 11104 32-33 -0.21215067936510223 11104 34-35 1.6258895173001804 11104 36-37 0.043459431135694615 11104 38-39 -1.3725567821074875 11104 40-41 0.39256961009040126 11104 42-43 0.45969728147555244 11104 44-45 -0.17127269252435795 11104 46-47 0.5909764613943302 11104 48-49 1.0610409245274077 11104 50-51 -0.619828236977277 11104 52-53 -0.19791658931718814 11104 54-55 -0.5466356450763925 11104 56-57 -1.4164042878895984 11104 58-59 -0.8918287490057075 11104 60-61 -1.29956609327375 11104 62-63 -1.3227982002302703 11104 64-65 -1.1743457172687144 11104 66-67 -0.93879755115724 11104 68-69 1.9994812876220287 11104 70-71 -0.8985677939429095 11104 72-73 -0.4782499984910764 11104 74-75 -0.2169244440834568 11104 76 -0.047698481527689296 11105 1 0.8740000102250995 11105 2 1.671698030639206 11105 3 -0.9502942407061816 11105 4 -1.8561829247343269 11105 5 -0.17726846339670743 11105 6 -0.6492427247452071 11105 7 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11105 68-69 1.276077032302883 11105 70-71 -0.8134614109641838 11105 72-73 -0.1697393601932049 11105 74-75 -1.1530946568494151 11105 76 0.01613130570635235 11106 1 -0.9000425429663892 11106 2 -0.8810679268076029 11106 3 -1.170719772621073 11106 4 -0.12894888218113465 11106 5 -0.19003442084351718 11106 6 -1.1441363417664832 11106 7 0.15950428640737258 11106 8 -2.3762477234398673 11106 9 0.48350211653194464 11106 10-11 0.8547579435962405 11106 12-13 -0.8948528417737762 11106 14-15 -0.28249093813028736 11106 16-17 -1.0595868868663203 11106 18-19 -0.16305951529670182 11106 20-21 0.049664465921836154 11106 22-23 -0.2597700125130231 11106 24-25 -0.9456255026568741 11106 26-27 -1.0873968260448663 11106 28-29 0.7300586900886685 11106 30-31 -1.614276540813261 11106 32-33 -0.7125762112799876 11106 34-35 -0.9243232486572666 11106 36-37 0.45473602688037573 11106 38-39 -1.8389397608308897 11106 40-41 -0.6501963473564025 11106 42-43 -3.233706973843592 11106 44-45 -0.7097833308222334 11106 46-47 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46-47 -0.7182678526188759 21612 48-49 -3.757877413335109E-5 21612 50-51 1.0617565025678424 21612 52-53 -0.4044463031836578 21612 54-55 1.2277590724584577 21612 56-57 -0.42832651826377344 21612 58-59 -0.7131787123219233 21612 60-61 -1.1480620580573166 21612 62-63 -0.8248158084474362 21612 64-65 -0.7124968544660746 21612 66-67 0.23618410694987446 21612 68-69 0.9827901650981836 21612 70-71 -0.3668363192547197 21612 72-73 -1.324911773986308 21612 74-75 1.7346529587045083 21612 76 -0.903898041322261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 3.0 16 6.0 17 14.0 18 45.0 19 78.0 20 191.0 21 484.0 22 801.0 23 1305.0 24 1775.0 25 2421.0 26 2938.0 27 3563.0 28 4138.0 29 4803.0 30 5693.0 31 6971.0 32 8637.0 33 11784.0 34 12837.0 35 6969.0 36 1050.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.327582150419577 24.81504718584163 8.724805897576662 43.132564766162126 2 16.002666457532744 47.549211826523404 12.3009960003137 24.147125715630146 3 16.52201192094531 28.90567813447663 31.8636934016522 22.70861654292586 4 12.038114159488675 39.480047577345864 22.282928359496516 26.198909903668945 5 12.228946370920308 43.01305762871371 29.296665664579713 15.461330335786268 6 22.39170783990798 51.32277207016443 14.700807779781977 11.584712310145608 7 20.871565628839566 28.84348939952422 16.66143831856325 33.62350665307296 8 19.769949676491734 48.36938762172407 14.644794457878572 17.21586824390563 9 20.50296708754869 16.1921940762816 15.128225237236295 48.176613598933415 10-11 20.079993203191883 32.13452451752465 29.519714011227805 18.265768268055655 12-13 20.473943560719935 19.982485262786412 29.89726430261283 29.646306873880825 14-15 19.68381978119649 19.616505679218896 18.106186362032233 42.59348817755238 16-17 30.44558014299345 31.415426039447368 17.56179173147555 20.577202086083627 18-19 30.416369850927698 23.509113604726394 18.057942789174778 28.01657375517113 20-21 19.5596481367718 21.700628700641772 17.911432940776663 40.82829022180977 22-23 19.43482295737645 32.58917484674605 18.251924660488584 29.724077535388922 24-25 30.270432770857568 32.33233560327813 18.19964186283608 19.19758976302822 26-27 30.450154887788045 33.305449174585334 19.269478609800412 16.974917327826212 28-29 19.406447902438387 32.945997346595995 19.948239015495616 27.699315735469998 30-31 18.985654063592694 22.806444233848566 40.26731152576713 17.940590176791606 32-33 30.257362071444444 21.09088057301946 21.52874900335917 27.123008352176925 34-35 30.08221469930856 21.367325865607068 19.724992484347837 28.825466950736534 36-37 31.7617995738952 30.708301201197273 19.27143921471238 18.258460010195147 38-39 19.288431123949444 23.783444652123332 30.107702563164157 26.820421660763067 40-41 18.490536442538954 21.596909965492 31.2127470459061 28.699806546062952 42-43 29.694454429593804 21.107806934413908 30.35587072317898 18.841867912813306 44-45 18.57803861084607 32.261100291476595 31.121335302652042 18.03952579502529 46-47 29.343947296184464 21.091329751774442 22.32984327411997 27.234879677921125 48-49 27.960994993660382 20.27044691057867 21.08217978379933 30.686378311961622 50-51 18.815271805194296 21.598023710248736 31.555936058138474 28.030768426418497 52-53 16.82961897915169 22.795895693091957 40.99732043657277 19.377164891183583 54-55 16.765786137216203 23.50503875462376 31.65592690864888 28.07324819951116 56-57 27.527546499013162 20.7719555073392 22.98809259283464 28.712405400812997 58-59 17.43926907215821 23.675422028481613 38.91419571141944 19.971113187940738 60-61 27.452389977387693 31.852640934816424 22.32475459761852 18.370214490177368 62-63 16.986680957298024 44.94359993203236 19.410642163462168 18.659076947207446 64-65 16.825911354516577 45.950697321813685 18.283294339080083 18.940096984589648 66-67 16.513521638542876 46.33432234958892 18.170886324127203 18.98126968774099 68-69 17.200386892702628 45.54354503509483 17.9676369482531 19.288431123949444 70-71 25.981282758440404 37.081574235037316 17.997046021932633 18.940096984589648 72-73 41.42071002823382 21.60017776848269 17.863771828923976 19.11534037435951 74-75 42.88431123949443 20.26023762531533 17.654593697308744 19.200857437881503 76 43.36727358280942 19.739370253702276 18.023187420758884 18.870168742729422 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 5.0 15 11.0 16 14.5 17 14.0 18 16.5 19 19.5 20 25.5 21 31.0 22 42.5 23 79.5 24 88.0 25 71.0 26 140.0 27 257.5 28 371.5 29 437.0 30 535.0 31 662.5 32 862.5 33 1033.0 34 1119.5 35 1369.5 36 1680.0 37 1827.0 38 2070.5 39 2552.5 40 3074.0 41 3613.5 42 3870.0 43 3994.5 44 4252.5 45 4737.5 46 5089.0 47 5462.5 48 6873.5 49 9660.5 50 11410.0 51 7199.0 52 2732.0 53 2235.5 54 1995.0 55 1811.5 56 1446.5 57 1115.5 58 966.0 59 834.0 60 640.5 61 495.5 62 412.0 63 386.0 64 321.5 65 257.0 66 219.5 67 208.0 68 216.0 69 207.0 70 175.0 71 160.0 72 141.0 73 128.5 74 113.5 75 92.0 76 78.5 77 72.5 78 64.5 79 49.0 80 50.5 81 46.5 82 35.5 83 30.0 84 23.5 85 13.0 86 8.5 87 8.0 88 8.0 89 6.0 90 6.5 91 5.5 92 2.0 93 3.0 94 4.5 95 4.0 96 3.0 97 4.5 98 16.5 99 900.5 100 1774.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013070699413125597 2 0.0013070699413125597 3 0.003921209823937679 4 0.0 5 0.0 6 0.0013070699413125597 7 0.0013070699413125597 8 0.0026141398826251194 9 0.0013070699413125597 10-11 6.535349706562798E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.535349706562798E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0019606049119688393 30-31 0.005881814735906518 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.003921209823937679 42-43 0.005881814735906518 44-45 0.0 46-47 0.005228279765250239 48-49 0.005228279765250239 50-51 0.0 52-53 0.0026141398826251194 54-55 0.0 56-57 0.0 58-59 0.0019606049119688393 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.003921209823937679 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 76507.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.6444900466624 #Duplication Level Percentage of deduplicated Percentage of total 1 80.5501072986132 45.62719751133883 2 9.899162378567967 11.214660096461762 3 4.190414657221312 7.120917040270824 4 2.0836698433209495 4.7211366280209655 5 1.2068209613032743 3.4179878965323436 6 0.560721785079724 1.905707974433712 7 0.38535200867618896 1.527964761394382 8 0.29535962341648014 1.3384396199040611 9 0.16613978817177008 0.8469813219705388 >10 0.6045642291806078 5.509299802632439 >50 0.032304958811177516 1.293999241899434 >100 0.011537485289706256 1.3122982210778098 >500 0.004614994115882502 1.4338557256198778 >1k 0.009229988231765004 12.729554158443019 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 4898 6.402028572548916 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1783 2.3305057053602938 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 1542 2.0155018495039667 No Hit CATAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 1516 1.9815180310298404 No Hit TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 549 0.7175813977805953 No Hit CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 548 0.7162743278392827 No Hit CAAAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 390 0.5097572771118983 No Hit CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 262 0.3424523246238906 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 122 0.1594625328401323 RNA PCR Primer, Index 30 (95% over 22bp) CACTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 117 0.15292718313356948 No Hit CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT 113 0.14769890336831926 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 89 0.11632922477681781 No Hit CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT 87 0.11371508489419269 No Hit ACTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 86 0.11240801495288012 No Hit CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 84 0.10979387507025501 No Hit AAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 80 0.10456559530500477 RNA PCR Primer, Index 30 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003921209823937679 0.0 2 0.0 0.0 0.0 0.003921209823937679 0.0 3 0.0 0.0 0.0 0.003921209823937679 0.0 4 0.0 0.0 0.0 0.006535349706562798 0.0 5 0.0 0.0 0.0 0.006535349706562798 0.0 6 0.0 0.0 0.0 0.009149489589187917 0.0 7 0.0 0.0 0.0 0.009149489589187917 0.0 8 0.0 0.0 0.0 0.009149489589187917 0.0 9 0.0 0.0 0.0 0.010456559530500478 0.0 10 0.0 0.0 0.0 0.010456559530500478 0.0 11 0.0 0.0 0.0 0.014377769354438156 0.0 12 0.0 0.0 0.0 0.016991909237063275 0.0 13 0.0 0.0 0.0 0.026141398826251192 0.0 14 0.0 0.0 0.0 0.03267674853281399 0.0 15 0.0 0.0 0.0 0.03267674853281399 0.0 16 0.0 0.0 0.0 0.037905028298064226 0.0 17 0.0 0.0 0.0 0.03921209823937679 0.0 18 0.0 0.0 0.0 0.03921209823937679 0.0 19 0.0 0.0 0.0 0.04444037800462703 0.0 20 0.0 0.0 0.0 0.04574744794593959 0.0 21 0.0 0.0 0.0 0.04836158782856471 0.0 22 0.0 0.0 0.0 0.05097572771118983 0.0 23 0.0 0.0 0.0 0.057511077417752625 0.0 24 0.0 0.0 0.0 0.05881814735906518 0.0 25 0.0 0.0 0.0 0.06273935718300286 0.0 26 0.0 0.0 0.0 0.06535349706562799 0.0 27 0.0 0.0 0.0 0.07581005659612845 0.0 28 0.0 0.0 0.0 0.09149489589187917 0.0 29 0.0 0.0 0.0 0.11110094501156757 0.0 30 0.0 0.0 0.0 0.13985648372044387 0.0 31 0.0 0.0 0.0 0.17122616231194532 0.0 32 0.0 0.0 0.0 0.207824120668697 0.0 33 0.0 0.0 0.0 0.24311500908413608 0.0 34 0.0 0.0 0.0 0.32284627550420225 0.0 35 0.0 0.0 0.0 0.4300260106918321 0.0 36 0.0 0.0 0.0 0.5881814735906519 0.0 37 0.0 0.0 0.0 0.8299894127334754 0.0 38 0.0 0.0 0.0 1.1175447998222385 0.0 39 0.0 0.0 0.0 1.4430052152090658 0.0 40 0.0 0.0 0.0 1.9566837021449017 0.0 41 0.0 0.0 0.0 2.428535950958736 0.0 42 0.0 0.0 0.0 2.9644346268968853 0.0 43 0.0 0.0 0.0 3.5578443802527873 0.0 44 0.0 0.0 0.0 4.221835910439568 0.0 45 0.0 0.0 0.0 4.885827440626348 0.0 46 0.0 0.0 0.0 5.411269557033997 0.0 47 0.0 0.0 0.0 6.0151358699204 0.0 48 0.0 0.0 0.0 6.530121426797548 0.0 49 0.0 0.0 0.0 7.1078463408577 0.0 50 0.0 0.0 0.0 7.583619799495471 0.0 51 0.0 0.0 0.0 8.06854274772243 0.0 52 0.0 0.0 0.0 8.601827283777954 0.0 53 0.0 0.0 0.0 9.106356281124603 0.0 54 0.0 0.0 0.0 9.664475146065065 0.0 55 0.0 0.0 0.0 10.144169814526775 0.0 56 0.0 0.0 0.0 10.617329133281922 0.0 57 0.0 0.0 0.0 11.064347053210817 0.0 58 0.0 0.0 0.0 11.53619930202465 0.0 59 0.0 0.0 0.0 11.879958696589854 0.0 60 0.0 0.0 0.0 12.256394839687871 0.0 61 0.0 0.0 0.0 12.630216842903264 0.0 62 0.0 0.0 0.0 12.994889356529468 0.0 63 0.0 0.0 0.0 13.383089129099298 0.0 64 0.0 0.0 0.0 13.798737370436692 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTAA 65 0.0 70.00001 46 ACTGCTT 20 7.881479E-5 70.0 53 TGCTTAA 15 0.0022169529 70.0 55 ACATTGA 35 3.6525307E-9 70.0 27 CGTCTAC 25 2.8157083E-6 70.0 48 TGACGGA 35 3.6525307E-9 70.0 31 ATCCGAG 30 1.0115582E-7 70.0 12 TTGACGG 30 1.0115582E-7 70.0 30 CCGACTT 25 2.8157083E-6 70.0 47 CGTAATC 50 0.0 70.0 48 CGTAAGA 20 7.881479E-5 70.0 41 CAAGAAT 15 0.0022169529 70.0 1 CGACTTC 15 0.0022169529 70.0 48 ATGCCGG 35 3.6525307E-9 70.0 30 ATTGACG 35 3.6525307E-9 70.0 29 CGTCATA 20 7.881479E-5 70.0 48 TAAGACG 15 0.0022169529 70.0 43 TGCCGAC 35 3.6525307E-9 70.0 45 CGGATAT 60 0.0 64.166664 34 TGCCGTA 105 0.0 63.333336 45 >>END_MODULE