FastQCFastQC Report
Wed 25 May 2016
SRR1781018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397443
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4994412.566330266226855No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG27240.6853812999599943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT150.002222276870.0123454
ATACGTA150.002222276870.0123455
TTACGGC150.002223386470.00352566
TATGCGC150.002224496369.994723
TGGCGGC308.3675495E-658.3362719
CGGTGCG252.3885338E-456.0028116
GCGCTGG509.786163E-1056.0028115
TGCGCGT252.3900194E-455.995775
ATACACG252.3900194E-455.995774
CGCGTCT452.6991984E-854.4403347
GGTCGCG200.006943866652.5092550
TACGTAA200.006947323752.50264456
GCGCGTC407.4757554E-752.4960376
ATGCGCG200.00695078252.4960374
GGCGCGT200.00695078252.4960375
CGGTATG200.00695078252.49603743
TCGCCGT4350.051.50332352
GTGCGCG352.08592E-549.9962234
GTCGCCG4500.049.7865551
GCCGTAT4450.049.5592954
Produced by FastQC (version 0.11.5)