FastQCFastQC Report
Wed 25 May 2016
SRR1781018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397443
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT162444.087126959085957No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT94302.372667275559011No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42121.0597746091892422No Hit
CATAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT33680.8474171139006096No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG27180.6838716495195537No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT13280.3341359641508342No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT11290.28406589120955705No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC8440.2123574952886326No Hit
CAAAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT8040.20229315901902917No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC7500.1887063050550645No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT4270.10743678967801672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTC750.065.3320448
AGGGGGG42700.063.20342370
CCGACTT950.062.63034447
GCCGACA453.8380676E-1062.22099346
TGCCGAC1300.059.229645
TCGAAAG308.3647865E-658.33952340
GCCGACT1150.057.82494446
CATATAC23950.057.5771521
GCCGTAT553.6379788E-1157.27159546
ATATACA24250.056.7205282
CGTAAGA252.389361E-455.99889441
ATATCGT950.055.26902437
CGTCTAC700.054.99891348
ATCGTAT900.054.45021439
GCCGTCT46600.054.22639546
CGTCTTC47150.054.11345748
CCGTCTT46200.054.01408447
TATGCCG49750.053.8180343
TCTTCTG46400.053.70583750
GTCTTCT46600.053.70065349