FastQCFastQC Report
Wed 25 May 2016
SRR1781017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51325
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3827674.5757428153921No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG19843.865562591329761No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT1830.35655138821237214No Hit
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC1310.2552362396492937No Hit
CTCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG1220.23770092547491478No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG740.144179249878227No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG660.12859230394544569No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580.1130053580126644No Hit
TCTTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGG580.1130053580126644No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580.1130053580126644No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540.10521188504627374No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530.10326351680467608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGC150.002205926770.05463419
GCGAGGA150.002205926770.05463423
GAGGCGG207.8643396E-569.986355
CTTTATA150.002214486569.986352
AGGCGGC150.002214486569.986356
GCGCTGC207.8643396E-569.9863515
GGAGGCG150.002214486569.986354
TCTTTAT150.002214486569.986351
TTTATAC150.002214486569.986353
GGCGCTG401.042099E-861.2380614
AAAAAGG1500.060.6548470
CTGGCGC308.276567E-658.3219612
TGGCGCT308.276567E-658.3219613
TCTGGCG252.3717775E-455.98908211
GTCTGGC252.3717775E-455.98908210
CGGCTGG252.3717775E-455.9890829
ATTAAAA2700.055.72987762
GTATCAT2650.055.46088457
CATTAAA2650.055.46088461
TATCATT2650.055.46088458
Produced by FastQC (version 0.11.5)