FastQCFastQC Report
Wed 25 May 2016
SRR1781017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51325
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT1219523.760350706283486No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT814115.861665854846565No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30535.948368241597661No Hit
CATAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT25604.987822698490015No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG21564.20068192888456No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT10211.9892839746712128No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT8471.6502679006332197No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT5531.0774476376035071No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3630.7072576716999514No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3560.6936190940087676No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3260.6351680467608378No Hit
ACTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG2790.5435947394057477No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC2700.5260594252313687No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT2170.4227959084266927RNA PCR Primer, Index 24 (95% over 23bp)
CTAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT1610.3136872868972236No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT1520.2961519727228446No Hit
TCATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1330.25913297613248903No Hit
AAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT1240.24159766195811005RNA PCR Primer, Index 24 (95% over 23bp)
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1190.23185582075012176No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1190.23185582075012176No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT1100.2143205065757428No Hit
CATAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT960.18704335119337556No Hit
CAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC940.18314661471018023RNA PCR Primer, Index 24 (95% over 21bp)
TCTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG900.17535314174378958No Hit
CACTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC840.16366293229420362No Hit
CATATACACATCTCCGAGCCCACGAGACGAGATAGCATCTCGTATGCCGT830.16171456405260595No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGA810.15781782756941062No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC810.15781782756941062No Hit
ACTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG790.1539210910862153No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC790.1539210910862153RNA PCR Primer, Index 24 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTAAGCCGT750.14612761811982464No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT750.14612761811982464No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT730.14223088163662934RNA PCR Primer, Index 24 (95% over 22bp)
CATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG720.14028251339503167No Hit
CATTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC720.14028251339503167No Hit
CATAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG700.13638577691183634No Hit
CATAAACACATCACCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT680.13248904042864101No Hit
CATAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGA640.12469556746225036No Hit
CACATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC630.12274719922065269No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGATAGCATCTCGTATGCCGT620.12079883097905503No Hit
CATATACACATCACCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT610.11885046273745739No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT570.11105698977106673No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT560.10910862152946907No Hit
CATAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTAAGCCGT550.1071602532878714No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGAATGCCGT550.1071602532878714No Hit
CATAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT540.10521188504627374No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC530.10326351680467608No Hit
CATAAACACATCTCCGAGCCCACGAGACGAGATAGCATCTCGTATGCCGT520.10131514856307841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGC207.823138E-570.0604951
ACTGCTA150.002205195470.06048653
GTCATAT150.002205195470.06048649
TATGCTA150.002205195470.06048653
CATCATC150.002205195470.06048651
GACATCT150.002205195470.06048649
TACTGCA150.002205195470.06048652
TCATATG150.002205195470.06048650
CTACTGA150.002205195470.06048651
CGACTAC150.002205195470.06048648
CACGTAT252.8043214E-669.9922139
ACACATA252.8043214E-669.992216
ACGGGGG252.8043214E-669.9922170
CACTAAT252.8043214E-669.992211
CTCGAAA207.861084E-569.992239
ACTCGTA700.069.992238
GTATGAC1250.069.992242
GCCCAGC150.002213752469.992220
AACGAGC150.002213752469.992213
AAACACC150.002213752469.99224
Produced by FastQC (version 0.11.5)