FastQCFastQC Report
Wed 25 May 2016
SRR1781016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242771
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4038916.63666582911468No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG16360.6738860901837535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT352.9729563E-759.980057
GCTGCGG252.3863299E-456.00444817
TCGCCGT2950.054.56936652
GTCGCCG2950.054.56936651
GCCGTAT2900.054.30347454
TGGTCGC2900.054.30347449
CGAAACG200.006937623552.5149931
CGCTGCG200.00694328152.50417316
CAGTCGC200.006954606652.4825445
TTACCAA200.006954606652.48254456
ATCTCGG3050.051.64344842
TCGGTGG3000.051.32683645
CGGTGGT3000.051.31626546
GATCTCG3100.050.81049341
CTCGGTG3050.050.48541344
TAGATCT3150.050.01428239
CGCCGTA3150.049.99367553
CGTATCA3150.049.98337656
ATCGCGT352.0866668E-549.9833766
TCGCGTG352.0866668E-549.9833767
Produced by FastQC (version 0.11.5)