FastQCFastQC Report
Wed 25 May 2016
SRR1781016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242771
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT144425.948815962367828No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT60582.495355705582627No Hit
CATAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT32041.3197622450786954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25281.0413105354428658No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG19000.7826305448344324No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT12240.5041788351986028No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT8430.34724081541864554No Hit
CAAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT7090.29204476646716454No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC4160.17135489823743363No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC4000.16476432522830156No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2990.12316133310815543No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2950.12151368985587241No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAGG150.002223433569.9948516
ACGTGAG150.002223433569.9948560
CGTAAGA252.8354807E-669.9948541
AGGGGGG36450.065.2075470
CATATAC19600.061.2454951
ATATACA19700.060.7569542
TAAGACG352.9676448E-759.99558643
AAACTCG950.058.95517336
AATCACG553.6379788E-1157.2803136
CCGTCTT38500.056.90490347
CGTCTTC39850.056.82141548
TCTTCTG39500.056.61608550
CGTATGC41300.056.60600741
ATATCGT1300.056.5459537
GCCGTCT38950.056.5170246
TATGCCG40950.056.4915743
TCGTATG42450.056.48568740
TCTGCTT39300.056.47058553
GTCTTCT39650.056.40190549
TCTCGTA41400.056.39635538
Produced by FastQC (version 0.11.5)