FastQCFastQC Report
Wed 25 May 2016
SRR1781015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279441
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5363519.193675945906293No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG24950.8928539477027351No Hit
TCTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGT2850.10198932869550281No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAC252.3881963E-455.99856612
TACGCGT252.3903098E-455.9885445
GACCGAC200.0069472652.49865713
CCATCCG200.0069472652.49865741
TCGCACC200.006952179652.4892646
AAACCGT200.006952179652.4892658
TGGTCGC4700.049.14768249
CGGTGGT4850.049.06213446
CTCGGTG4800.048.84417344
TCGGTGG4950.048.77789745
GCCGTAT4550.048.460354
TCGCCGT4500.048.2209952
GTCGCCG4700.047.65835651
ACGTACT305.8724626E-446.66547413
CGCCGTA4650.046.66547453
GACACGT305.8776466E-446.65712410
ACGCGTC305.8776466E-446.6571246
ATCTCGG5000.046.1905542
GATCTCG4950.045.9584241
CCGTATC4800.045.93632555
Produced by FastQC (version 0.11.5)