FastQCFastQC Report
Wed 25 May 2016
SRR1781015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279441
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT174976.261429067316536TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT96423.4504600255510107TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39931.4289241736180447No Hit
CATAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT35271.2621626747685557No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG27320.9776661263021532TruSeq Adapter, Index 5 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT14930.5342809394469673TruSeq Adapter, Index 5 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT11200.40080016890864256RNA PCR Primer, Index 5 (95% over 23bp)
CAAAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT7450.26660368378298105RNA PCR Primer, Index 5 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6430.2301022398288011RNA PCR Primer, Index 5 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC5910.21149366055804264TruSeq Adapter, Index 5 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT4320.15459435086476214TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT3550.12703933925229297TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC3080.11022004644987672RNA PCR Primer, Index 5 (95% over 21bp)
ACTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG3020.10807290268786612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG44750.064.835570
CATATAC22650.062.4370351
GCCGTAA401.0635631E-861.2476546
ACTCCGA1450.060.34251411
ATATACA23450.059.99772
TGCCGTA700.059.997745
GCCGTAT553.6379788E-1157.2705346
ATCGTAT1600.056.87281839
CATAAAC7700.056.3715631
ACGTTAC252.388348E-455.99785216
TGACGAC252.388348E-455.99785245
CCGACGT252.388348E-455.99785213
CCCGACG252.388348E-455.99785212
GCCGTCT50200.055.565646
TATGCCG53000.055.4695743
GTATGCC53500.055.3436742
ATGCCGT53250.055.34060344
TGCCGTC52500.055.2645545
CCGTCTT49750.055.2240147
CGTATGC54000.055.0256741
Produced by FastQC (version 0.11.5)